WB
Western blot analysis of ACP5 using anti-ACP5 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: HepG2 whole cell lysates, Lane 2: A431 whole cell lysates, Lane 3: Jurkat whole cell lysates, Lane 4: Hela whole cell lysates, Lane 5: K562 whole cell lysates, Lane 6: Raji whole cell lysates, Lane 7: rat lung tissue lysates, Lane 8: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ACP5 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
IHC analysis of ACP5 using anti-ACP5 antibody. ACP5 was detected in a paraffin-embedded section of human spleen tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-ACP5 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.IHC
IHC analysis of ACP5 using anti-ACP5 antibody. ACP5 was detected in a paraffin-embedded section of mouse lung tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-ACP5 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.ICC/IF
ICC/IF analysis of ACP5 using anti-ACP5 antibody. ACP5 was detected in an immunocytochemical section of MCF-7 cells. The section was incubated with rabbit anti-ACP5 Antibody at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI (Blue).FC
Flow Cytometry analysis of U251 cells using anti-ACP5 antibody. Overlay histogram showing U251 cells stained with anti-ACP5 antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ACP5 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Product Name | Anti-ACP5 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human Acid phosphatase/ACP5 recombinant protein (Position: N39-P325). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:500-2000 IHC:1:50-400 ICC/IF:1:50-400 FC:1:50-200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ACP5 |
|---|---|
| Gene Synonyms | HPAP TRAP TRAcP TRACP5a TRACP5b TrATPase |
| Gene Full Name | acid phosphatase 5, tartrate resistant |
| Gene Summary | This gene encodes an iron containing glycoprotein which catalyzes the conversion of orthophosphoric monoester to alcohol and orthophosphate. It is the most basic of the acid phosphatases and is the only form not inhibited by L(+)-tartrate. [provided by RefSeq, Aug 2008] |
| Alternative Names | ACP5 Tartrate resistant acid ATPase TR AP TRACP TRAP TrATPase Type 5 acid phosphatase |
| Molecular Weight(MW) | 37kDa(Observed MW 37kDa) |
| Cellular Localization | Lysosome |
WB
Western blot analysis of ACP5 using anti-ACP5 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: HepG2 whole cell lysates, Lane 2: A431 whole cell lysates, Lane 3: Jurkat whole cell lysates, Lane 4: Hela whole cell lysates, Lane 5: K562 whole cell lysates, Lane 6: Raji whole cell lysates, Lane 7: rat lung tissue lysates, Lane 8: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ACP5 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
IHC analysis of ACP5 using anti-ACP5 antibody. ACP5 was detected in a paraffin-embedded section of human spleen tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-ACP5 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IHC
IHC analysis of ACP5 using anti-ACP5 antibody. ACP5 was detected in a paraffin-embedded section of mouse lung tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-ACP5 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
ICC/IF
ICC/IF analysis of ACP5 using anti-ACP5 antibody. ACP5 was detected in an immunocytochemical section of MCF-7 cells. The section was incubated with rabbit anti-ACP5 Antibody at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI (Blue).
FC
Flow Cytometry analysis of U251 cells using anti-ACP5 antibody. Overlay histogram showing U251 cells stained with anti-ACP5 antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ACP5 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Application Notes | WB:1:500-2000 IHC:1:50-400 ICC/IF:1:50-400 FC:1:50-200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
