WB
Western blot analysis of HMGCR using anti-HMGCR antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: Caco-2 whole cell lysates, Lane 2: RT4 whole cell lysates, Lane 3: THP-1 whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-HMGCR antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
IHC analysis of HMGCR using anti-HMGCR antibody. HMGCR was detected in a paraffin-embedded section of human breast cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-HMGCR Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.IF-P
IF analysis using anti- HMGCR antibody detected in paraffin-embedded section of human breast cancer tissue. The tissue section were stained using the cy3-conjugated Anti-rabbit IgG Secondary Antibody (red) and counterstained with DAPI (blue).FC
Flow Cytometry analysis of HEL cells using anti-HMGCR antibody. Overlay histogram showing HEL cells stained with anti-HMGCR antibody (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-HMGCR Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Product Name | Anti-HMGCR Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human RAP1GAP recombinant protein (Position: D11-L661). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCIF-PIHCWB |
| WB:1:500-2000 IHC:1:50-400 IF-P:1:50-400 FC:1:50-200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | HMGCR |
|---|---|
| Gene Synonyms | MYPLG LDLCQ3 LGMDR28 |
| Gene Full Name | 3-hydroxy-3-methylglutaryl-CoA reductase |
| Gene Summary | HMG-CoA reductase is the rate-limiting enzyme for cholesterol synthesis and is regulated via a negative feedback mechanism mediated by sterols and non-sterol metabolites derived from mevalonate, the product of the reaction catalyzed by reductase. Normally in mammalian cells this enzyme is suppressed by cholesterol derived from the internalization and degradation of low density lipoprotein (LDL) via the LDL receptor. Competitive inhibitors of the reductase induce the expression of LDL receptors in the liver, which in turn increases the catabolism of plasma LDL and lowers the plasma concentration of cholesterol, an important determinant of atherosclerosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008] |
| Alternative Names | HMG CoA reductase HMGCR |
| Molecular Weight(MW) | 97kDa(Observed MW 97kDa) |
| Cellular Localization | Endoplasmic reticulum membrane |
WB
Western blot analysis of HMGCR using anti-HMGCR antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: Caco-2 whole cell lysates, Lane 2: RT4 whole cell lysates, Lane 3: THP-1 whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-HMGCR antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
IHC analysis of HMGCR using anti-HMGCR antibody. HMGCR was detected in a paraffin-embedded section of human breast cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-HMGCR Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IF-P
IF analysis using anti- HMGCR antibody detected in paraffin-embedded section of human breast cancer tissue. The tissue section were stained using the cy3-conjugated Anti-rabbit IgG Secondary Antibody (red) and counterstained with DAPI (blue).
FC
Flow Cytometry analysis of HEL cells using anti-HMGCR antibody. Overlay histogram showing HEL cells stained with anti-HMGCR antibody (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-HMGCR Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Application Notes | WB:1:500-2000 IHC:1:50-400 IF-P:1:50-400 FC:1:50-200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
