WB
Western blot analysis of various lysates, using ABCG1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 20s.IHC
Immunohistochemistry analysis of paraffinembedded Human colon carcinoma usingABCG1 Rabbit pAb at dilution of1:100 (40x lens). Microwave antigen retrievalperformed with 0.01M PBS Buffer (pH 7.2)prior to IHC staining.IF-P
Immunofluorescence analysis of Rat lungusing ABCG1 Rabbit pAb at dilutionof 1:100. Blue: DAPI for nuclear staining.| Product Name | ABCG1 Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein (or fragment).This information is considered to be commercially sensitive. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IF-PIHCWB |
| WB:1:500-1:1000 IHC:1:50-1:200 IF-P:1:50-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ABCG1 |
|---|---|
| Gene Synonyms | ABC8 WHITE1 |
| Gene Full Name | ATP binding cassette subfamily G member 1 |
| Gene Summary | The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the White subfamily. It is involved in macrophage cholesterol and phospholipids transport, and may regulate cellular lipid homeostasis in other cell types. Six alternative splice variants have been identified. [provided by RefSeq, Jul 2008] |
| Alternative Names | ABC8 WHITE1 ABCG1 |
| Molecular Weight(MW) | 76kDa(Observed band size:75kDa) |
| Cellular Localization | Endoplasmic reticulum membrane, Golgi apparatus membrane, Multi-pass membrane protein. |
WB
Western blot analysis of various lysates, using ABCG1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 20s.
IHC
Immunohistochemistry analysis of paraffinembedded Human colon carcinoma usingABCG1 Rabbit pAb at dilution of1:100 (40x lens). Microwave antigen retrievalperformed with 0.01M PBS Buffer (pH 7.2)prior to IHC staining.
IF-P
Immunofluorescence analysis of Rat lungusing ABCG1 Rabbit pAb at dilutionof 1:100. Blue: DAPI for nuclear staining.| Application Notes | WB:1:500-1:1000 IHC:1:50-1:200 IF-P:1:50-1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3. |
