WB
Western blot analysis of Phospho-Cytosolic Phospholipase A2 (S505) on different lysates with Rabbit anti-Phospho-Cytosolic Phospholipase A2 (S505) antibody at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa cell lysate, the membrane treated with λpp for 1 hour Lysates/proteins at 20 µg/Lane. Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.WB
Western blot analysis of Phospho-Cytosolic Phospholipase A2 (S505) on RAW264.7 cell lysates with Rabbit anti-Phospho-Cytosolic Phospholipase A2 (S505) antibody at 1/1,000 dilution. Lysates/proteins at 20 µg/Lane. Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.DB
Dot blot analysis of Phospho-Cytosolic Phospholipase A2 (S505) on different proteins with Rabbit anti-Phospho-Cytosolic Phospholipase A2 (S505) antibody at 1/1,000 dilution. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution for 1 hour at room temperature. Lane 1: Unmodified Cytosolic Phospholipase A2 (negative), Lane 2: Phospho-Cytosolic Phospholipase A2 (S505) (positive), Proteins loading: 50ng, 25ng, 5ng, 1ng; Blocking and dilution buffer: 5% NFDM/TBST; Exposure time: 6 seconds.| Product Name | Phospho-Cytosolic Phospholipase A2 (S505) Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic phospho-peptide corresponding to residues surrounding Ser505 of human cPLA2 aa 491-540. |
| Modification | p-S505 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | DBWB |
| WB:1000:2000 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PLA2G4A |
|---|---|
| Gene Synonyms | GURDP cPLA2 PLA2G4 cPLA2-alpha |
| Gene Full Name | phospholipase A2 group IVA |
| Gene Summary | This gene encodes a member of the cytosolic phospholipase A2 group IV family. The enzyme catalyzes the hydrolysis of membrane phospholipids to release arachidonic acid which is subsequently metabolized into eicosanoids. Eicosanoids, including prostaglandins and leukotrienes, are lipid-based cellular hormones that regulate hemodynamics, inflammatory responses, and other intracellular pathways. The hydrolysis reaction also produces lysophospholipids that are converted into platelet-activating factor. The enzyme is activated by increased intracellular Ca(2+) levels and phosphorylation, resulting in its translocation from the cytosol and nucleus to perinuclear membrane vesicles. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2015] |
| Molecular Weight(MW) | 85 kDa(Observed band size:100kDa) |
| Cellular Localization | Cytoplasm, Golgi apparatus membrane, Nucleus envelope. |
WB
Western blot analysis of Phospho-Cytosolic Phospholipase A2 (S505) on different lysates with Rabbit anti-Phospho-Cytosolic Phospholipase A2 (S505) antibody at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa cell lysate, the membrane treated with λpp for 1 hour Lysates/proteins at 20 µg/Lane. Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
WB
Western blot analysis of Phospho-Cytosolic Phospholipase A2 (S505) on RAW264.7 cell lysates with Rabbit anti-Phospho-Cytosolic Phospholipase A2 (S505) antibody at 1/1,000 dilution. Lysates/proteins at 20 µg/Lane. Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
DB
Dot blot analysis of Phospho-Cytosolic Phospholipase A2 (S505) on different proteins with Rabbit anti-Phospho-Cytosolic Phospholipase A2 (S505) antibody at 1/1,000 dilution. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution for 1 hour at room temperature. Lane 1: Unmodified Cytosolic Phospholipase A2 (negative), Lane 2: Phospho-Cytosolic Phospholipase A2 (S505) (positive), Proteins loading: 50ng, 25ng, 5ng, 1ng; Blocking and dilution buffer: 5% NFDM/TBST; Exposure time: 6 seconds.| Application Notes | WB:1000:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
