WB
Western Blot, Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, Jurkat whole cell lysate, Raji whole cell lysate, U87 whole cell lysate, HepG2 whole cell lysate, Rat liver tissue, Mouse brain tissue, Mouse kidney tissue, All lanes: PNCK antibody at 3.1µg/ml, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution.IHC
IHC image of PNCK Antibody diluted at 1:200 and staining in paraffin-embedded human appendix tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.| Product Name | PNCK Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant Human Calcium/calmodulin-dependent protein kinase type 1B protein (1-115AA) |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:500-1:5000 IHC:1:200-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Symbol | PNCK |
|---|---|
| Gene Synonyms | BSTK3 CaMK1b |
| Gene Full Name | pregnancy up-regulated nonubiquitous CaM kinase |
| Gene Summary | PNCK is a member of the calcium/calmodulin-dependent protein kinase family of protein serine/threonine kinases (see CAMK1; MIM 604998) (Gardner et al., 2000 [PubMed 10673339]).[supplied by OMIM, Mar 2008] |
| Molecular Weight(MW) | 39/41/28/47kDa(Observed MW 39kDa) |
| Cellular Localization | Cytoplasm. Nucleus. |
WB
Western Blot, Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, Jurkat whole cell lysate, Raji whole cell lysate, U87 whole cell lysate, HepG2 whole cell lysate, Rat liver tissue, Mouse brain tissue, Mouse kidney tissue, All lanes: PNCK antibody at 3.1µg/ml, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution.
IHC
IHC image of PNCK Antibody diluted at 1:200 and staining in paraffin-embedded human appendix tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.| Application Notes | WB:1:500-1:5000 IHC:1:200-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Storage Buffer | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
