WB
Western Blot,
Positive WB detected in: Hela whole cell lysate(30µg), MCF-7 whole cell lysate(30µg), SY5Y whole cell lysate(30µg), 293 whole cell lysate(30µg),HT29 whole cell lysate(30µg),Mouse Heart tissue lysate(30µg),Rat Heart tissue lysate(30µg),
All lanes: S1PR2 antibody at 1:1000,
Secondary,
Goat polyclonal to rabbit IgG at 1/20000 dilution,
Exposure time: 120s.