WB
Western blot analysis using Histone H3(citrulline R2/R8/R17) antibody against Lane 1:Untreated HEK-293T (human embryonic kidney) transfected with PADI4 expression vector containing a myc-His-tag, whole cell lysate at 20 µg.Lane 2:HEK-293T transfected with PADI4 expression vector containing a myc-His-tag treated with 10 mM calcium chloride and 10 μM lonomycin for 4 hours, whole cell lysate at 20 µg.Lane 3:Untreated NIH/3T3 (mouse embryonic fibroblast) transfected with PADI4 expression vector containing a myc-His-tag, whole cell lysate at 20 µg.Lane 4:NIH/3T3 transfected with PADI4 expression vector containing a myc-His-tag treated with 10 mM calcium chloride and 10 μM lonomycin for 4 hours, whole cell lysate at 20 µg. 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:2000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.| Product Name | Anti-Histone H3(citrulline R2/R8/R17) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:1000 |
|
| Species Reactivity | All |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Synonyms | H3C1 H3C2 H3C3 H3C4 H3C6 H3C7 H3C8 H3C10 H3C11 H3C12 |
|---|---|
| Gene Summary | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015] |
| Alternative Names | Histone H3.1 |
| Molecular Weight(MW) | 17 kDa |
| Cellular Localization | Nucleus |
| SwissProt ID | P68431 |
|---|
WB
Western blot analysis using Histone H3(citrulline R2/R8/R17) antibody against Lane 1:Untreated HEK-293T (human embryonic kidney) transfected with PADI4 expression vector containing a myc-His-tag, whole cell lysate at 20 µg.Lane 2:HEK-293T transfected with PADI4 expression vector containing a myc-His-tag treated with 10 mM calcium chloride and 10 μM lonomycin for 4 hours, whole cell lysate at 20 µg.Lane 3:Untreated NIH/3T3 (mouse embryonic fibroblast) transfected with PADI4 expression vector containing a myc-His-tag, whole cell lysate at 20 µg.Lane 4:NIH/3T3 transfected with PADI4 expression vector containing a myc-His-tag treated with 10 mM calcium chloride and 10 μM lonomycin for 4 hours, whole cell lysate at 20 µg. 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:2000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.| Application Notes | WB:1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% PC300,40% Glycerol. |
