WB
15% SDS-PAGE gel.Sample loading: 10ng /lane. Transfer the proteins onto a PVDF membrane (OM790002), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:5000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:10000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 30S.| Product Name | Anti-IL36RA antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:5000 |
|
| Species Reactivity | Human |
| Concentration | 1μg/μl |
| Purification | Affinity purified |
| Gene Symbol | Il36rn |
|---|---|
| Gene Synonyms | Il1f5 If36rn Il-1h3 Il1hy1 IL-36Ra Fil1delta |
| Gene Full Name | interleukin 36 receptor antagonist |
| Gene Summary | Predicted to enable cytokine activity and interleukin-1 receptor binding activity. Acts upstream of or within negative regulation of interleukin-6 production. Predicted to be located in cytoplasm. Predicted to be active in extracellular space. Human ortholog(s) of this gene implicated in pustular psoriasis 14. Orthologous to human IL36RN (interleukin 36 receptor antagonist). [provided by Alliance of Genome Resources, Apr 2025] |
WB
15% SDS-PAGE gel.Sample loading: 10ng /lane. Transfer the proteins onto a PVDF membrane (OM790002), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:5000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:10000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 30S.| Application Notes | WB:1:5000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide,40% Glycerol. |
