ICC/IF
Immunofluorescence analysis of NIH3T3 cells using Histone H3 (citrulline R2) antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Omnimabs® 594-Phalloidin.Cells are fixed in 4% paraformaldehyde at room temperature for 15 minutes. Then, they are permeabilized with a PBS (OM750003) solution containing 0.1% Triton X-100(OM750021) at room temperature for 15 minutes. Subsequently, the cells are blocked with 10% non - immune goat serum(OM760028) at room temperature for 1 hour.The cells are incubated overnight at 4°C with the primary antibody diluted 1:100 in PBS. The secondary antibody, Omnimabs® 488 Goat Ant-Rabbit IgG(H&L) (Green,OM643486), is diluted at a ratio of 1:400 and incubated with the cells for 1 hour.Nuclear DNA is labeled with DAPI (Blue,OM643160). F-actin is stained with Omnimabs® 594-Phalloidin (Red,OM750007) diluted 1:100 for 30 minutes.| Product Name | Anti-Histone H3 (citrulline R2) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide conjugated with KLH |
| Clonality | polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IF |
| ICC/IF:1:100-1:500 |
|
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | H3C1; H3C2; H3C3; H3C4; H3C6; H3C7; H3C8; H3C10; H3C11; H3C12;H3-3A; H3-3B; H3-4;H3-5; H3C15; H3C14; H3C13 |
|---|---|
| Gene Synonyms | BRYLIB2 CELE_T10C6.12 CG5825 dH3.3A Dmel\CG5825 Dmel_CG5825 H3 H3-53 H3-614 H3.2 H3.2-614 H3.3 H3.3A H3.3B H3/l H3/M H3/n H3/o H3c13 H3c14 H3c15 H3c2 H3c3 H3c4 H3c6 H3c7 H3F2 H3F3B H3FL H3FM H3FN His-3.3A His3.3 HIST1H3B HIST2H3A HIST2H3C Hist2h3c2 Hist2h3c2-ps Hist2h3ca1 PH3 |
| Gene Full Name | H3 clustered histone 1 |
| Gene Summary | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015] |
| Alternative Names | CG5825-PA CG5825-PC CG5825-PD H3 histone family member 3B H3 histone family member L H3 histone family member M H3 histone family 2 H3 histone family 3B (H3.3B) H3-clustered histone 13 H3-clustered histone 14 H3-clustered histone 15 H3-clustered histone 2 H3-clustered histone 3 H3-clustered histone 4 H3-clustered histone 6 H3-clustered histone 7 H3K56me3 H3L-like histone H3Lys18me3 H3Lys56me3 His3.3A-PA His3.3A-PC His3.3A-PD histone histone 1 H3b histone 2 H3a histone 2 H3c histone 2 H3c2 histone 2 H3ca2 histone cluster 1 H3 family member b histone cluster 1 H3b histone cluster 2 H3 family member a histone cluster 2 H3 family member c histone cluster 2 H3C family member 2 histone cluster 2 H3a histone cluster 2 H3c histone cluster 2 H3c2 histone cluster 2 H3c2 pseudogene histone H3.3 histone H3.3 histone H3/l histone H3/m histone H3/o Tri-methyl H3 Tri-methyl Histone H3 Trimethyl H3 Trimethyl Histone H3 |
| Cellular Localization | Nucleus, Chromosome. |
| SwissProt ID | P68431 |
|---|
ICC/IF
Immunofluorescence analysis of NIH3T3 cells using Histone H3 (citrulline R2) antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Omnimabs® 594-Phalloidin.Cells are fixed in 4% paraformaldehyde at room temperature for 15 minutes. Then, they are permeabilized with a PBS (OM750003) solution containing 0.1% Triton X-100(OM750021) at room temperature for 15 minutes. Subsequently, the cells are blocked with 10% non - immune goat serum(OM760028) at room temperature for 1 hour.The cells are incubated overnight at 4°C with the primary antibody diluted 1:100 in PBS. The secondary antibody, Omnimabs® 488 Goat Ant-Rabbit IgG(H&L) (Green,OM643486), is diluted at a ratio of 1:400 and incubated with the cells for 1 hour.Nuclear DNA is labeled with DAPI (Blue,OM643160). F-actin is stained with Omnimabs® 594-Phalloidin (Red,OM750007) diluted 1:100 for 30 minutes.| Application Notes | ICC/IF:1:100-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide,40% Glycerol. |
