ELISA
Microtiter plate wells were coated with 100 ng/well coating antigen and incubated with serial dilutions of the antibody. Following HRP-conjugated secondary antibody and substrate development, OD450 was measured. The absorbance decreased progressively with increasing antibody dilution, yielding a sigmoidal titration curve that demonstrates the high antigen-binding activity of the polyclonal antibody.| Product Name | Anti-Caspase-3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide conjugated with KLH |
| Clonality | polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISA |
| ELISA:1:1000-1:10000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CASP3 |
|---|---|
| Gene Synonyms | A830040C14Rik AC-3 CASP-3 Caspase-3 CC3 CPP-32 CPP32 CPP32-beta CPP32B Lice mldy SCA-1 Yama |
| Gene Full Name | caspase 3 |
| Gene Summary | The protein encoded by this gene is a cysteine-aspartic acid protease that plays a central role in the execution-phase of cell apoptosis. The encoded protein cleaves and inactivates poly(ADP-ribose) polymerase while it cleaves and activates sterol regulatory element binding proteins as well as caspases 6, 7, and 9. This protein itself is processed by caspases 8, 9, and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. [provided by RefSeq, Aug 2017] |
| Alternative Names | apopain Apopain precursor CASP-3 caspase 3 apoptosis related cysteine protease Caspase 3 apoptosis related cysteine protease (ICE-like cysteine protease) caspase 3 apoptosis-related cysteine peptidase caspase 3 apoptosis-related cysteine protease Caspase3 CPP-32 CPP32beta cysteine protease CPP32 cysteine protease p32-beta ICE3 IRP PARP cleavage protease procaspase3 protein Yama SCA-1 SREBP cleavage activity 1 |
| Cellular Localization | Cytoplasm. |
| SwissProt ID | P42574 |
|---|
ELISA
Microtiter plate wells were coated with 100 ng/well coating antigen and incubated with serial dilutions of the antibody. Following HRP-conjugated secondary antibody and substrate development, OD450 was measured. The absorbance decreased progressively with increasing antibody dilution, yielding a sigmoidal titration curve that demonstrates the high antigen-binding activity of the polyclonal antibody.| Application Notes | ELISA:1:1000-1:10000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide,40% Glycerol. |
