ELISA
Microtiter plate wells were coated with 100 ng/well coating antigen and incubated with serial dilutions of the antibody. Following HRP-conjugated secondary antibody and substrate development, OD450 was measured. The absorbance decreased progressively with increasing antibody dilution, yielding a sigmoidal titration curve that demonstrates the high antigen-binding activity of the polyclonal antibody.| Product Name | Anti-PPARG antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant Human PPARG protein |
| Clonality | polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISA |
| ELISA:1:1000-1:10000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PPARG |
|---|---|
| Gene Synonyms | CIMT1 FPLD3 GLM1 NR1C3 PPAR-GAMMA PPAR-gamma2 PPARG1 PPARG2 PPARG5 PPARGAMMA PPARgamma2 |
| Gene Full Name | peroxisome proliferator activated receptor gamma |
| Gene Summary | This gene encodes a member of the peroxisome proliferator-activated receptor (PPAR) subfamily of nuclear receptors. PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate transcription of various genes. Three subtypes of PPARs are known: PPAR-alpha, PPAR-delta, and PPAR-gamma. The protein encoded by this gene is PPAR-gamma and is a regulator of adipocyte differentiation. Additionally, PPAR-gamma has been implicated in the pathology of numerous diseases including obesity, diabetes, atherosclerosis and cancer. Alternatively spliced transcript variants that encode different isoforms have been described. [provided by RefSeq, Jul 2008] |
| Alternative Names | HUMPPARG ligand-activated nuclear transcription factor ligand-activated transcription factor nuclear hormone receptor nuclear hormone receptor PPARG nuclear receptor nuclear receptor family member Pparg Nuclear receptor subfamily 1 group C member 3 nuclear receptor transcription factor OTTHUMP00000185032 OTTHUMP00000185036 peroxisome proliferative activated receptor gamma peroxisome proliferator peroxisome proliferator activated receptor peroxisome proliferator activated receptor gamma peroxisome proliferator activated receptor gamma 2 peroxisome proliferator activated receptor gamma 4 peroxisome proliferator activated receptor gamma-1 peroxisome proliferator activatived receptor gamma peroxisome proliferator activator receptor gamma peroxisome proliferator-activated receptor gamma 1 peroxisome proliferator-activated receptor gamma 1-a peroxisome proliferator-activated receptor gamma 1-b peroxisome proliferator-activated receptor gamma 2 peroxisome proliferator-activated receptor-gamma 5 peroxisome proliferator-activated receptor-gamma splicing PPAR gamma PPAR gamma 1 PPAR gamma 3 PPAR-gamma PPAR-gamma-1a PPAR-gamma-1b Pparg PPARgamma PPARy transcription factor |
| Cellular Localization | Nucleus, Cytoplasm. |
| SwissProt ID | P37231 |
|---|
ELISA
Microtiter plate wells were coated with 100 ng/well coating antigen and incubated with serial dilutions of the antibody. Following HRP-conjugated secondary antibody and substrate development, OD450 was measured. The absorbance decreased progressively with increasing antibody dilution, yielding a sigmoidal titration curve that demonstrates the high antigen-binding activity of the polyclonal antibody.| Application Notes | ELISA:1:1000-1:10000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide,40% Glycerol. |
