WB
Western blot analysis using Anti-CD23-Rat antibody against Recombinant CD23-Rat protein. 12% SDS-PAGE gel.Sample loading: 10ng /lane. Transfer the proteins onto a PVDF membrane (OM790002), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:5000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:10000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 30S.ELISA
Microtiter plate wells were coated with 100 ng/well coating antigen and incubated with serial dilutions of the antibody. Following HRP-conjugated secondary antibody and substrate development, OD450 was measured. The absorbance decreased progressively with increasing antibody dilution, yielding a sigmoidal titration curve that demonstrates the high antigen-binding activity of the polyclonal antibody.| Product Name | Anti-CD23 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant Rat CD23 protein |
| Clonality | polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| ELISA:1:1000-1:10000 WB:1:5000 |
|
| Species Reactivity | Rat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | FCER2 |
|---|---|
| Gene Synonyms | CD23 FCE2 CD23A IGEBF CLEC4J FCErII BLAST-2 |
| Gene Full Name | Fc epsilon receptor II |
| Gene Summary | The protein encoded by this gene is a B-cell specific antigen, and a low-affinity receptor for IgE. It has essential roles in B cell growth and differentiation, and the regulation of IgE production. This protein also exists as a soluble secreted form, then functioning as a potent mitogenic growth factor. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.[provided by RefSeq, Jul 2011] |
| Alternative Names | Blast 2 antibody
BLAST-2 antibody
Blast2 antibody
C type lectin domain family 4 member J antibody
C-type lectin domain family 4 antibody
C-type lectin domain family 4 member J antibody
C-type lectin domain family 4 member J antibody CD 23 antibody CD 23A antibody CD23 antibody CD23 antigen antibody CD23A antibody CLEC 4J antibody CLEC4J antibody Fc epsilon receptor II antibody Fc epsilon RII antibody Fc fragment of IgE antibody Fc fragment of IgE low affinity II receptor for antibody Fc fragment of IgE receptor II antibody Fc fragment of IgE low affinity II receptor for (CD23) antibody Fc of IgE antibody Fc of IgE low affinity II receptor for (CD23) antibody Fc receptor IgE low affinity II alpha polypeptide antibody Fc receptor IgE low affinity II alpha polypeptide isoform CRA_a antibody Fc-epsilon-RII antibody FCE 2 antibody FCE2 antibody FCER 2 antibody Fcer2 antibody FCER2_HUMAN antibody FCER2A antibody FceRII antibody IgE binding factor antibody IgE receptor lymphocyte antibody IgE-binding factor antibody IGEBF antibody Immunoglobulin E binding factor antibody Immunoglobulin E receptor antibody Immunoglobulin E receptor low affinity II antibody Immunoglobulin E-binding factor antibody Immunoglobulin epsilon chain antibody LEUKOCYTE ANTIGEN CD23 antibody Low Affinity IgE Receptor antibody Low affinity immunoglobulin epsilon Fc receptor antibody Low affinity immunoglobulin epsilon Fc receptor membrane bound form antibody Low affinity immunoglobulin epsilon Fc receptor soluble form antibody Ly-42 antibody Ly42 antibody Lymphocyte antigen CD23 antibody Lymphocyte IgE receptor antibody MGC93219 antibody |
| Cellular Localization | Cell membrane, Secreted. |
| SwissProt ID | Q63097 |
|---|
WB
Western blot analysis using Anti-CD23-Rat antibody against Recombinant CD23-Rat protein. 12% SDS-PAGE gel.Sample loading: 10ng /lane. Transfer the proteins onto a PVDF membrane (OM790002), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:5000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:10000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 30S.
ELISA
Microtiter plate wells were coated with 100 ng/well coating antigen and incubated with serial dilutions of the antibody. Following HRP-conjugated secondary antibody and substrate development, OD450 was measured. The absorbance decreased progressively with increasing antibody dilution, yielding a sigmoidal titration curve that demonstrates the high antigen-binding activity of the polyclonal antibody.| Application Notes | ELISA:1:1000-1:10000 WB:1:5000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide,40% Glycerol. |
