ELISA
Microtiter plate wells were coated with 100 ng/well coating antigen and incubated with serial dilutions of the antibody. Following HRP-conjugated secondary antibody and substrate development, OD450 was measured. The absorbance decreased progressively with increasing antibody dilution, yielding a sigmoidal titration curve that demonstrates the high antigen-binding activity of the polyclonal antibody.ICC/IF
Immunofluorescence analysis of A549 cells using IFNA2 antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Omnimabs® 594-Phalloidin.Cells are fixed in 4% paraformaldehyde at room temperature for 15 minutes. Then, they are permeabilized with a PBS (OM750003) solution containing 0.1% Triton X-100(OM750021) at room temperature for 15 minutes. Subsequently, the cells are blocked with 10% non - immune goat serum(OM760028) at room temperature for 1 hour.The cells are incubated overnight at 4°C with the primary antibody diluted 1:100 in PBS. The secondary antibody, Omnimabs® 488 Goat Ant-Rabbit IgG(H&L) (Green,OM643486), is diluted at a ratio of 1:400 and incubated with the cells for 1 hour.Nuclear DNA is labeled with DAPI (Blue,OM643160). F-actin is stained with Omnimabs® 594-Phalloidin (Red,OM750007) diluted 1:100 for 30 minutes| Product Name | Anti-IFNA2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant Human IFNA2 protein |
| Clonality | polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IF |
| ELISA:1:1000-1:10000 ICC/IF:1:100-1:500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | IFNA2 |
|---|---|
| Gene Synonyms | Gm12597 If1ai1 If1ai14 If1ai7 If1ai9 Ifa1 Ifa2 IFL IFN IFN-ALPHA IFN-alpha-2 IFN-alpha1 IFN-alphaA IFN-alphaD IFNA Ifna1 IFNA13 Ifna1l1 IFNA2B IFNA@ Ifnaa leIF A leIF D MuIFN-alpha-A |
| Gene Full Name | interferon alpha 2 |
| Gene Summary | This gene is a member of the alpha interferon gene cluster on chromosome 9. The encoded cytokine is a member of the type I interferon family that is produced in response to viral infection as a key part of the innate immune response with potent antiviral, antiproliferative and immunomodulatory properties. This cytokine, like other type I interferons, binds a plasma membrane receptor made of IFNAR1 and IFNAR2 that is ubiquitously expressed, and thus is able to act on virtually all body cells. The encoded protein is effective in reducing the symptoms and duration of the common cold and in treating many types of cancer, including some hematological malignancies and solid tumors. A deficiency of type I interferon in the blood is thought to be a hallmark of severe COVID-19 and may provide a rationale for a combined therapeutic approach. [provided by RefSeq, Aug 2020] |
| Alternative Names | alpha-2a interferon cytokine ifa IFN alpha 1/13 IFN alpha 1b IFN α IFN-alpha-1 IFN-alpha-1/13 IFN-ALPHA-2 IFN-ALPHAA IFNA IFNA2 IFNA2A IFNA2B IFNA2C IFNAalphaIFN-alpha-1/13 IFNalpha IFNα interferon 1ai1 interferon 1ai14 interferon 1ai7 interferon 1ai9 Interferon alpha 13 (IFNA13) interferon alpha 1b interferon alpha 2a interferon alpha 2b interferon alpha A interferon alpha family gene 1 interferon alpha family gene 2 Interferon alpha leukocyte (IFL) interferon alpha-1/13 Interferon alpha-1/13 (IFN-alpha-1/13) interferon alpha-D Interferon alpha-D (IFN alphaD) Interferon alpha1 (IFNA1) interferon-alpha 1 interferon-alpha 1-like 1 interferon-alpha1 LEIF A MGC138207 MGC138505 MGC138507 Mouse interferon alpha-2 novel interferon alpha (Ifna) family member |
| Cellular Localization | Secreted. |
| SwissProt ID | P01563 |
|---|
ELISA
Microtiter plate wells were coated with 100 ng/well coating antigen and incubated with serial dilutions of the antibody. Following HRP-conjugated secondary antibody and substrate development, OD450 was measured. The absorbance decreased progressively with increasing antibody dilution, yielding a sigmoidal titration curve that demonstrates the high antigen-binding activity of the polyclonal antibody.
ICC/IF
Immunofluorescence analysis of A549 cells using IFNA2 antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Omnimabs® 594-Phalloidin.Cells are fixed in 4% paraformaldehyde at room temperature for 15 minutes. Then, they are permeabilized with a PBS (OM750003) solution containing 0.1% Triton X-100(OM750021) at room temperature for 15 minutes. Subsequently, the cells are blocked with 10% non - immune goat serum(OM760028) at room temperature for 1 hour.The cells are incubated overnight at 4°C with the primary antibody diluted 1:100 in PBS. The secondary antibody, Omnimabs® 488 Goat Ant-Rabbit IgG(H&L) (Green,OM643486), is diluted at a ratio of 1:400 and incubated with the cells for 1 hour.Nuclear DNA is labeled with DAPI (Blue,OM643160). F-actin is stained with Omnimabs® 594-Phalloidin (Red,OM750007) diluted 1:100 for 30 minutes| Application Notes | ELISA:1:1000-1:10000 ICC/IF:1:100-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide,40% Glycerol. |
