WB
Western blot analysis using CD4 antibody against B16(1),Nih3t3(2) cell,Mouse lung tissue(3) . 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:15000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.ELISA
Microtiter plate wells were coated with 100 ng/well coating antigen and incubated with serial dilutions of the antibody. Following HRP-conjugated secondary antibody and substrate development, OD450 was measured. The absorbance decreased progressively with increasing antibody dilution, yielding a sigmoidal titration curve that demonstrates the high antigen-binding activity of the polyclonal antibody.| Product Name | Anti-CD4 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant Human CD4 protein |
| Clonality | polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| ELISA:1:1000-1:10000 WB:1:1000-1:2000 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CD4 |
|---|---|
| Gene Synonyms | CD3-DELTA CD3-GAMMA CD3-ZETA CD3DELTA CD3epsilon CD3GAMMA CD3H CD3Q CD3Z CD3ZETA CD4mut IMD17 IMD18 IMD19 IMD25 IMD79 Leu-3 OKT4D T3D T3E T3G T3Z T4 TCRE TCRZ |
| Gene Full Name | CD4 molecule |
| Gene Summary | This gene encodes the CD4 membrane glycoprotein of T lymphocytes. The CD4 antigen acts as a coreceptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell in the context of class II MHC molecules. The CD4 antigen is also a primary receptor for entry of the human immunodeficiency virus through interactions with the HIV Env gp120 subunit. This gene is expressed not only in T lymphocytes, but also in B cells, macrophages, granulocytes, as well as in various regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been identified in this gene. [provided by RefSeq, May 2020] |
| Alternative Names | CD247 CD247 antigen zeta subunit CD3 antigen delta subunit CD3 delta CD3-epsilon CD3d antigen delta polypeptide (TiT3 complex) CD3d molecule delta (CD3-TCR complex) CD3e antigen epsilon polypeptide (TiT3 complex) CD3e molecule epsilon (CD3-TCR complex) CD3g antigen gamma polypeptide (TiT3 complex) CD3g molecule epsilon (CD3-TCR complex) CD3g molecule gamma (CD3-TCR complex) CD3Z antigen zeta polypeptide (TiT3 complex) CD3zeta chain CD4 antigen (p55) CD4 antigen p55 CD4 receptor component of the TCR/CD3 complex FLJ18683 Leu-2 MAL OKT3 delta chain OKT8 T-cell antigen T cell co-receptor T-cell antigen Leu2 T-cell antigen receptor complex epsilon subunit of T3 T-cell antigen receptor complex gamma subunit of T3 T-cell antigen receptor complex zeta subunit of CD3 T-cell receptor T3 delta chain T-cell receptor T3 gamma chain T-cell receptor T3 zeta chain T-cell surface antigen T3/Leu-4 epsilon chain T-cell surface antigen T4/Leu-3 T4 surface glycoprotein precursor T8 T-cell antigen TCR zeta TCR zeta chain tissue factor-targeting CAR1 dimer tissue factor-targeting CAR1 monomer |
| Molecular Weight(MW) | 51kDa（observed：55kDa) |
| Cellular Localization | Cell membrane. |
| SwissProt ID | P01730 |
|---|
WB
Western blot analysis using CD4 antibody against B16(1),Nih3t3(2) cell,Mouse lung tissue(3) . 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:15000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.
ELISA
Microtiter plate wells were coated with 100 ng/well coating antigen and incubated with serial dilutions of the antibody. Following HRP-conjugated secondary antibody and substrate development, OD450 was measured. The absorbance decreased progressively with increasing antibody dilution, yielding a sigmoidal titration curve that demonstrates the high antigen-binding activity of the polyclonal antibody.| Application Notes | ELISA:1:1000-1:10000 WB:1:1000-1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide,40% Glycerol. |
