ELISA
Microtiter plate wells were coated with 100 ng/well coating antigen and incubated with serial dilutions of the antibody. Following HRP-conjugated secondary antibody and substrate development, OD630 was measured. The absorbance decreased progressively with increasing antibody dilution, yielding a sigmoidal titration curve that demonstrates the high antigen-binding activity of the polyclonal antibody.| Product Name | Anti-Phospho-Histone H2A(Thr121) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide conjugated with KLH |
| Modification | p-Thr121 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISA |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | H2AC13 |
|---|---|
| Gene Synonyms | H2A/c H2AFC HIST1H2AI |
| Gene Full Name | H2A clustered histone 13 |
| Gene Summary | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2A family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the small histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015] |
| Alternative Names | H2A histone family member P H2A.1 H2A.1b H2A/n H2A/p H2A1_HUMAN H2AF H2AFC H2AFD ANDHIST1H2AL H2AFD H2AFI H2AFN ANDHIST1H2AG H2AFN H2AFP HIST1H2AI HIST1H2AJ HIST1H2AK HIST1H2AL HIST1H2AM Histone 1 H2ag Histone cluster 1 H2ag Histone H2A type 1 Histone H2A.c/d/i/n/p Histone H2A/p pH2A/f H2A histone family member A H2A histone family member M H2A.1 H2A.2 H2A/a H2A/m H2A1B_HUMAN H2AFA H2AFM HIST1H2AB HIST1H2AE Histone 1 H2ab Histone 1 H2ae Histone cluster 1 H2ab Histone cluster 1 H2ae Histone H2A type 1-B/E Histone H2A.2 Histone H2A/a Histone H2A/m Histone H2AE H2a 615 H2A H2A GL101 H2A histone family member A H2A.1 H2A.2 H2A/a H2A/m H2A/O H2A/q H2A1B_HUMAN H2AFA H2AFE H2AFL H2AFM H2AFO H2AFQ HIST1H2AE HIST1H2AJ HIST2H2AA HIST2H2AA3 HIST2H2AB HIST2H2AC Histone 1 H2ae Histone 2 H2aa3 Histone 2 H2ab Histone 2 H2ac Histone H2A type 1 B Histone H2A type 1 C Histone H2A type 1 E Histone H2A type 1 J Histone H2A type 1-B/E Histone H2A.2 Histone H2A/a Histone H2A/m MGC74460 H2a 615 H2A H2A GL101 H2A histone family member A H2A.1 H2A.2 H2A/a H2A/m H2A/O H2A/q H2A1B_HUMAN H2AFA H2AFE H2AFL H2AFM H2AFO H2AFQ HIST1H2AE HIST1H2AJ HIST2H2AA HIST2H2AA3 HIST2H2AB HIST2H2AC Histone 1 H2ae Histone 2 H2aa3 Histone 2 H2ab Histone 2 H2ac Histone H2A type 1 B Histone H2A type 1 C Histone H2A type 1 E Histone H2A type 1 J Histone H2A type 1-B/E Histone H2A.2 Histone H2A/a Histone H2A/m MGC74460 H2a 615 H2A H2A GL101 H2A histone family member A H2A.1 H2A.2 H2A/a H2A/m H2A/O H2A/q H2A1B_HUMAN H2AFA H2AFE H2AFL H2AFM H2AFO H2AFQ HIST1H2AE HIST1H2AJ HIST2H2AA HIST2H2AA3 HIST2H2AB HIST2H2AC Histone 1 H2ae Histone 2 H2aa3 Histone 2 H2ab Histone 2 H2ac Histone H2A type 1 B Histone H2A type 1 C Histone H2A type 1 E Histone H2A type 1 J Histone H2A type 1-B/E Histone H2A.2 Histone H2A/a Histone H2A/m MGC74460 H2a 615 H2A H2A GL101 H2A histone family member A H2A.1 H2A.2 H2A/a H2A/m H2A/O H2A/q H2A1B_HUMAN H2AFA H2AFE H2AFL H2AFM H2AFO H2AFQ HIST1H2AE HIST1H2AJ HIST2H2AA HIST2H2AA3 HIST2H2AB HIST2H2AC Histone 1 H2ae Histone 2 H2aa3 Histone 2 H2ab Histone 2 H2ac Histone H2A type 1 B Histone H2A type 1 C Histone H2A type 1 E Histone H2A type 1 J Histone H2A type 1-B/E Histone H2A.2 Histone H2A/a Histone H2A/m MGC74460 |
| Molecular Weight(MW) | 14kDa |
| Cellular Localization | Nucleus. Chromosome. |
| SwissProt ID | Q7L7L0 |
|---|
ELISA
Microtiter plate wells were coated with 100 ng/well coating antigen and incubated with serial dilutions of the antibody. Following HRP-conjugated secondary antibody and substrate development, OD630 was measured. The absorbance decreased progressively with increasing antibody dilution, yielding a sigmoidal titration curve that demonstrates the high antigen-binding activity of the polyclonal antibody.| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide,40% Glycerol. |
