ELISA
Coat the plate with 100ng immunizing antigen. The phospho-specific antibody,pbs only can specifically recognize phosphorylated peptide and enable quantitative detection over a wide dynamic range. Non-phosphorylated peptide shows no cross-reactivity, and the background signal remains low, indicating that the phospho-specific antibody,pbs only possesses high specificity and an excellent signal-to-noise ratio. Black line: phospho-peptide; Rad line: non-phospho peptide| Product Name | Anti-Phospho-ZAP70(Tyr319) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide conjugated with KLH. |
| Modification | p-Tyr319 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISA |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ZAP70 |
|---|---|
| Gene Synonyms | SRK STD TZK STCD IMD48 ADMIO2 ZAP-70 |
| Gene Full Name | zeta chain of T cell receptor associated protein kinase 70 |
| Gene Summary | This gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| Alternative Names | 70 kDa zeta associated protein 70 kDa zeta-associated protein EC 2.7.10.2 FLJ17670 FLJ17679 Selective T cell defect SRK STD Syk related tyrosine kinase Syk-related tyrosine kinase Truncated ZAP kinase Tyrosine protein kinase ZAP70 Tyrosine-protein kinase ZAP-70 TZK ZAP 70 ZAP70 ZAP70_HUMAN Zeta chain associated protein kinase 70kD Zeta chain associated protein kinase 70kDa Zeta chain associated protein kinase 70kDa isoform 1 Zeta chain associated protein kinase 70kDa isoform 2 Zeta chain TCR associated protein kinase 70kD Zeta chain TCR associated protein kinase 70kDa |
| Molecular Weight(MW) | 70kDa |
| Cellular Localization | Cell membrane, Cytoplasm, Membrane |
| SwissProt ID | P43403 |
|---|
ELISA
Coat the plate with 100ng immunizing antigen. The phospho-specific antibody,pbs only can specifically recognize phosphorylated peptide and enable quantitative detection over a wide dynamic range. Non-phosphorylated peptide shows no cross-reactivity, and the background signal remains low, indicating that the phospho-specific antibody,pbs only possesses high specificity and an excellent signal-to-noise ratio. Black line: phospho-peptide; Rad line: non-phospho peptide| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide,40% Glycerol. |
