ELISA
Coat the plate with 100ng immunizing antigen. The phospho-specific antibody,pbs only can specifically recognize phosphorylated peptide and enable quantitative detection over a wide dynamic range. Non-phosphorylated peptide shows no cross-reactivity, and the background signal remains low, indicating that the phospho-specific antibody,pbs only possesses high specificity and an excellent signal-to-noise ratio. Black line: phospho-peptide; Rad line: non-phospho peptide| Product Name | Anti-Phospho-Chk1 (S317) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide conjugated with KLH. |
| Modification | p-S317 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISA |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CHEK1 |
|---|---|
| Gene Synonyms | CHK1 OZEMA21 |
| Gene Full Name | checkpoint kinase 1 |
| Gene Summary | The protein encoded by this gene belongs to the Ser/Thr protein kinase family. It is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. This protein acts to integrate signals from ATM and ATR, two cell cycle proteins involved in DNA damage responses, that also associate with chromatin in meiotic prophase I. Phosphorylation of CDC25A protein phosphatase by this protein is required for cells to delay cell cycle progression in response to double-strand DNA breaks. Several alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Oct 2011] |
| Alternative Names | C85740 Cell cycle checkpoint kinase Checkpoint S. pombe homolog of 1 Checkpoint kinase 1 Checkpoint kinase 1 homolog (S. pombe) CHEK 1 Chek1 Chk 1 Chk1 CHK1 checkpoint homolog (S. pombe) CHK1_HUMAN EC 2.7.11.1 rad27 Serine/threonine protein kinase Chk1 Serine/threonine-protein kinase CHK1 STT3 subunit of the oligosaccharyltransferase complex homolog A (S. cerevisiae) |
| Molecular Weight(MW) | 54 kDa |
| Cellular Localization | Chromosome,Cytoplasm,Cytoskeleton,Nucleus. |
| SwissProt ID | O14757 |
|---|
ELISA
Coat the plate with 100ng immunizing antigen. The phospho-specific antibody,pbs only can specifically recognize phosphorylated peptide and enable quantitative detection over a wide dynamic range. Non-phosphorylated peptide shows no cross-reactivity, and the background signal remains low, indicating that the phospho-specific antibody,pbs only possesses high specificity and an excellent signal-to-noise ratio. Black line: phospho-peptide; Rad line: non-phospho peptide| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide,40% Glycerol. |
