ELISA
Coat the plate with 100ng immunizing antigen. The phospho-specific antibody,pbs only can specifically recognize phosphorylated peptide and enable quantitative detection over a wide dynamic range. Non-phosphorylated peptide shows no cross-reactivity, and the background signal remains low, indicating that the phospho-specific antibody,pbs only possesses high specificity and an excellent signal-to-noise ratio. Black line: phospho-peptide; Rad line: non-phospho peptide| Product Name | Anti-Phospho-PKC-pan (Thr497) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide conjugated with KLH. |
| Modification | p-Thr497 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISA |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PRKCA/PRKCB/PRKCG/PRKCH |
|---|---|
| Gene Synonyms | PKCB PRKCB1 PRKCB2 PKCI(2) PKCbeta PKC-beta PKCC PKCG SCA14 PKCI(3) PKCgamma PKC-gamma PKCL PKC-L PRKCL uORF2 nPKC-eta AAG6 PKCA PKCI+/- PKCalpha PKC-alpha |
| Gene Full Name | protein kinase C alpha/protein kinase C beta/protein kinase C gamma/protein kinase C eta |
| Gene Summary | Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This protein kinase has been reported to be involved in many different cellular functions, such as B cell activation, apoptosis induction, endothelial cell proliferation, and intestinal sugar absorption. Studies in mice also suggest that this kinase may also regulate neuronal functions and correlate fear-induced conflict behavior after stress. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008] |
| Alternative Names | AAG6 Aging associated gene 6 aPKC KPCA_HUMAN PKC alpha PKC-A PKC-alpha PKCA PRKACA PRKCA Protein Kinase C alpha Protein kinase C alpha type KPCG_HUMAN MGC57564 OTTHUMP00000067291 PKC-gamma PKCC PKCG PRKCG Protein kinase C gamma Protein kinase C gamma polypeptide Protein kinase C gamma type Protein kinase C gamma SCA 14 SCA14 |
| Molecular Weight(MW) | 77 kDa, 78 kDa |
| Cellular Localization | Cytoplasm, Membrane, Nucleus,Cell membrane, Cell projection, Cytoplasm, Membrane, Synapse, Synaptosome. |
| SwissProt ID | P05771 |
|---|
ELISA
Coat the plate with 100ng immunizing antigen. The phospho-specific antibody,pbs only can specifically recognize phosphorylated peptide and enable quantitative detection over a wide dynamic range. Non-phosphorylated peptide shows no cross-reactivity, and the background signal remains low, indicating that the phospho-specific antibody,pbs only possesses high specificity and an excellent signal-to-noise ratio. Black line: phospho-peptide; Rad line: non-phospho peptide| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide,40% Glycerol. |
