ELISA
Coat the plate with 100ng immunizing antigen. The phospho-specific antibody,pbs only can specifically recognize phosphorylated peptide and enable quantitative detection over a wide dynamic range. Non-phosphorylated peptide shows no cross-reactivity, and the background signal remains low, indicating that the phospho-specific antibody,pbs only possesses high specificity and an excellent signal-to-noise ratio. Black line: phospho-peptide; Rad line: non-phospho peptide| Product Name | Anti-Phospho-PDHK1 (Tyr243) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide conjugated with KLH. |
| Modification | p-Tyr243 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISA |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PDK1 |
|---|---|
| Gene Full Name | pyruvate dehydrogenase kinase 1 |
| Gene Summary | Pyruvate dehydrogenase (PDH) is a mitochondrial multienzyme complex that catalyzes the oxidative decarboxylation of pyruvate and is one of the major enzymes responsible for the regulation of homeostasis of carbohydrate fuels in mammals. The enzymatic activity is regulated by a phosphorylation/dephosphorylation cycle. Phosphorylation of PDH by a specific pyruvate dehydrogenase kinase (PDK) results in inactivation. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jun 2013] |
| Alternative Names | [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 1 mitochondrial HGNC:8809 Mitochondrial pyruvate dehydrogenase kinase isoenzyme 1 PDH kinase 1 Pdk1 PDK1_HUMAN Pyruvate dehydrogenase kinase isoform 1 Pyruvate dehydrogenase kinase isoenzyme 1 |
| Molecular Weight(MW) | 49 kDa |
| Cellular Localization | Mitochondrion. |
| SwissProt ID | Q15118 |
|---|
ELISA
Coat the plate with 100ng immunizing antigen. The phospho-specific antibody,pbs only can specifically recognize phosphorylated peptide and enable quantitative detection over a wide dynamic range. Non-phosphorylated peptide shows no cross-reactivity, and the background signal remains low, indicating that the phospho-specific antibody,pbs only possesses high specificity and an excellent signal-to-noise ratio. Black line: phospho-peptide; Rad line: non-phospho peptide| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide,40% Glycerol. |
