ELISA
Coat the plate with 100ng immunizing antigen. The phospho-specific antibody,pbs only can specifically recognize phosphorylated peptide and enable quantitative detection over a wide dynamic range. Non-phosphorylated peptide shows no cross-reactivity, and the background signal remains low, indicating that the phospho-specific antibody,pbs only possesses high specificity and an excellent signal-to-noise ratio. Black line: phospho-peptide; Rad line: non-phospho peptide| Product Name | Anti-Phospho-GRF-1 (Tyr1105) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide conjugated with KLH. |
| Modification | p-Tyr1105 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISA |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ARHGAP35 |
|---|---|
| Gene Synonyms | GRF-1 GRLF1 P190A P190-A p190RhoGAP p190ARhoGAP |
| Gene Full Name | Rho GTPase activating protein 35 |
| Gene Summary | The human glucocorticoid receptor DNA binding factor, which associates with the promoter region of the glucocorticoid receptor gene (hGR gene), is a repressor of glucocorticoid receptor transcription. The amino acid sequence deduced from the cDNA sequences show the presence of three sequence motifs characteristic of a zinc finger and one motif suggestive of a leucine zipper in which 1 cysteine is found instead of all leucines. The GRLF1 enhances the homologous down-regulation of wild-type hGR gene expression. Biochemical analysis suggests that GRLF1 interaction is sequence specific and that transcriptional efficacy of GRLF1 is regulated through its interaction with specific sequence motif. The level of expression is regulated by glucocorticoids. [provided by RefSeq, Jul 2008] |
| Alternative Names | ARHGAP35 GAP associated protein p190 Glucocorticoid receptor DNA binding factor 1 Glucocorticoid receptor DNA-binding factor 1 Glucocorticoid receptor repression factor 1 GRF 1 GRF-1 GRF1 GRLF 1 GRLF1 GRLF1_HUMAN KIAA1722 MGC10745 p190 A p190-A P190A P190A rat homolog of p190ARhoGAP p190RhoGAP Rho GAP p190A Rho GTPase-activating protein 35 |
| Molecular Weight(MW) | 171 kDa |
| Cellular Localization | Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Membrane, Nucleus. |
| SwissProt ID | Q9NRY4 |
|---|
ELISA
Coat the plate with 100ng immunizing antigen. The phospho-specific antibody,pbs only can specifically recognize phosphorylated peptide and enable quantitative detection over a wide dynamic range. Non-phosphorylated peptide shows no cross-reactivity, and the background signal remains low, indicating that the phospho-specific antibody,pbs only possesses high specificity and an excellent signal-to-noise ratio. Black line: phospho-peptide; Rad line: non-phospho peptide| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide,40% Glycerol. |
