ELISA
Coat the plate with 100ng immunizing antigen. The phospho-specific antibody,pbs only can specifically recognize phosphorylated peptide and enable quantitative detection over a wide dynamic range. Non-phosphorylated peptide shows no cross-reactivity, and the background signal remains low, indicating that the phospho-specific antibody,pbs only possesses high specificity and an excellent signal-to-noise ratio. Black line: phospho-peptide; Rad line: non-phospho peptide| Product Name | Anti-Phospho-WNK1 (Thr60) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide conjugated with KLH. |
| Modification | p-Thr60 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISA |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | WNK1 |
|---|---|
| Gene Synonyms | KDP PSK p65 HSN2 HSAN2 PRKWNK1 PPP1R167 |
| Gene Full Name | WNK lysine deficient protein kinase 1 |
| Gene Summary | This gene encodes a member of the WNK subfamily of serine/threonine protein kinases. The encoded protein may be a key regulator of blood pressure by controlling the transport of sodium and chloride ions. Mutations in this gene have been associated with pseudohypoaldosteronism type II and hereditary sensory neuropathy type II. Alternatively spliced transcript variants encoding different isoforms have been described but the full-length nature of all of them has yet to be determined.[provided by RefSeq, May 2010] |
| Alternative Names | Erythrocyte 65 kDa protein HSAN2 HSN2 hWNK1 KDP KIAA0344 Kinase deficient protein MGC163339 MGC163341 p65 PPP1R167 PRKWNK1 Prostate derived sterile 20 like kinase Protein kinase lysine deficient 1 Protein kinase lysine-deficient 1 Protein kinase with no lysine 1 Protein phosphatase 1 regulatory subunit 167 PSK Serine/threonine protein kinase WNK1 1 Serine/threonine protein kinase WNK1 2 Serine/threonine protein kinase WNK1 Serine/threonine-protein kinase WNK1 With no K WNK lysine deficient protein kinase 1 WNK lysine deficient protein kinase 1 isoform WNK1 WNK1_HUMAN |
| Molecular Weight(MW) | 251 kDa |
| Cellular Localization | Cytoplasm, Cytoskeleton, Nucleus. |
| SwissProt ID | Q9H4A3 |
|---|
ELISA
Coat the plate with 100ng immunizing antigen. The phospho-specific antibody,pbs only can specifically recognize phosphorylated peptide and enable quantitative detection over a wide dynamic range. Non-phosphorylated peptide shows no cross-reactivity, and the background signal remains low, indicating that the phospho-specific antibody,pbs only possesses high specificity and an excellent signal-to-noise ratio. Black line: phospho-peptide; Rad line: non-phospho peptide| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide,40% Glycerol. |
