ELISA
Coat the plate with 100ng immunizing antigen. The phospho-specific antibody,pbs only can specifically recognize phosphorylated peptide and enable quantitative detection over a wide dynamic range. Non-phosphorylated peptide shows no cross-reactivity, and the background signal remains low, indicating that the phospho-specific antibody,pbs only possesses high specificity and an excellent signal-to-noise ratio. Black line: phospho-peptide; Rad line: non-phospho peptide| Product Name | Anti-Phospho-PAK1 (Thr212) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide conjugated with KLH. |
| Modification | p-Thr212 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISA |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PAK1 |
|---|---|
| Gene Synonyms | IDDMSSD p65-PAK PAKalpha alpha-PAK |
| Gene Full Name | p21 (RAC1) activated kinase 1 |
| Gene Summary | This gene encodes a family member of serine/threonine p21-activating kinases, known as PAK proteins. These proteins are critical effectors that link RhoGTPases to cytoskeleton reorganization and nuclear signaling, and they serve as targets for the small GTP binding proteins Cdc42 and Rac. This specific family member regulates cell motility and morphology. Mutations in this gene have been associated with macrocephaly, seizures, and speech delay. Overexpression of this gene is also reported in many cancer types, and particularly in breast cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2020] |
| Alternative Names | ADRB2 Alpha PAK Alpha-PAK MGC130000 MGC130001 p21 activated kinase 1 p21 protein (Cdc42/Rac) activated kinase 1 p21-activated kinase 1 p21/Cdc42/Rac1 activated kinase 1 (yeast Ste20 related) p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog yeast) p65 PAK p65-PAK P68-PAK PAK alpha PAK-1 Pak1 PAK1_HUMAN Paka PAKalpha Protein kinase MUK2 Rac/p21-activated kinase Serine/threonine-protein kinase PAK 1 STE20 homolog yeast |
| Molecular Weight(MW) | 61 kDa |
| Cellular Localization | Cell junction,Cell membrane,Cell projection,Chromosome,Cytoplasm,Cytoskeleton,Membrane,Nucleus. |
| SwissProt ID | Q13153 |
|---|
ELISA
Coat the plate with 100ng immunizing antigen. The phospho-specific antibody,pbs only can specifically recognize phosphorylated peptide and enable quantitative detection over a wide dynamic range. Non-phosphorylated peptide shows no cross-reactivity, and the background signal remains low, indicating that the phospho-specific antibody,pbs only possesses high specificity and an excellent signal-to-noise ratio. Black line: phospho-peptide; Rad line: non-phospho peptide| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide,40% Glycerol. |
