ELISA
Coat the plate with 100ng immunizing antigen. The phospho-specific antibody,pbs only can specifically recognize phosphorylated peptide and enable quantitative detection over a wide dynamic range. Non-phosphorylated peptide shows no cross-reactivity, and the background signal remains low, indicating that the phospho-specific antibody,pbs only possesses high specificity and an excellent signal-to-noise ratio. Black line: phospho-peptide; Rad line: non-phospho peptide| Product Name | Anti-Phospho-AMPK beta 1 (Ser181) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide conjugated with KLH. |
| Modification | p-Ser181 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISA |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PRKAB1 |
|---|---|
| Gene Synonyms | AMPK HAMPKb |
| Gene Full Name | protein kinase AMP-activated non-catalytic subunit beta 1 |
| Gene Summary | The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit may be a positive regulator of AMPK activity. The myristoylation and phosphorylation of this subunit have been shown to affect the enzyme activity and cellular localization of AMPK. This subunit may also serve as an adaptor molecule mediating the association of the AMPK complex. [provided by RefSeq, Jul 2008] |
| Alternative Names | 1300015D22Rik antibody 5'-AMP-activated protein kinase subunit beta-1 antibody 5'-AMP-activated protein kinase beta-1 subunit antibody AAKB1_HUMAN antibody AMP-activated protein kinase beta subunit antibody AMP-ACTIVATED PROTEIN KINASE NONCATALYTIC BETA-1 antibody AMP-activated noncatalytic beta-1 antibody AMPK antibody AMPK beta 1 chain antibody AMPK subunit beta-1 antibody AMPK-BETA-1 antibody AMPKb antibody AU021155 antibody E430008F22 antibody HAMPKb antibody MGC17785 antibody PRKAB1 antibody Protein kinase AMP activated non catalytic subunit beta 1 antibody protein kinase AMP-activated beta 1 non-catalytic subunit antibody protein kinase AMP-activated noncatalytic beta-1 antibody |
| Molecular Weight(MW) | 30 kDa |
| Cellular Localization | Cytosol, nucleoplasm, nucleus, cytoplasm, nucleotide-activated protein kinase complex. |
| SwissProt ID | Q9Y478 |
|---|
ELISA
Coat the plate with 100ng immunizing antigen. The phospho-specific antibody,pbs only can specifically recognize phosphorylated peptide and enable quantitative detection over a wide dynamic range. Non-phosphorylated peptide shows no cross-reactivity, and the background signal remains low, indicating that the phospho-specific antibody,pbs only possesses high specificity and an excellent signal-to-noise ratio. Black line: phospho-peptide; Rad line: non-phospho peptide| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide,40% Glycerol. |
