SDS-PAGE
SDS-PAGE for Rat Anti-Human GPC3 Antibody .FC
Flow-cytometry using anti-human GPC3 antibody. Untransfected cells and Transfected cells were stained with an anti-human GPC3 monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Rat IgG H&L Polyclonal Antibody, FITC and cells analysed on a NovoCyte Flow Cytometer.Bioactivity
Detects recombinant human Glypican-3/GPC3 in indirect ELISA.Bioactivity
Detects recombinant mouse Glypican-3/GPC3 in indirect ELISA.WB
Western blot analysis was performed using anti-GPC3 monoclonal antibody at 1ug/mL on various samples.Lane 1:recombinant human GPC3 Lane 2:negative control| Product Name | Rat Anti-Human GPC3 Recombinant Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1,k |
| Clone Number | V9K3 |
| Host Species | Rat |
| Tested Applications | ELISAFCMWB |
| ELISA:1:2000-1:20000 WB:1:500-1:2000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A/G |
| Target | GPC3, MXR7, GTR2-2, Glypican-3, Intestinal protein OCI-5, OCI5 |
|---|---|
| Function | Rat Anti-Human GPC3 Antibody (YP7)[YP7] (HW310053) is arat monoclonalantibody detectingGPC3inELISA, FCM, WB. Suitable forHuman.HighlightsMulti-Application— Validated across multiple applications.Flow Cytometry— Validated for flow cytometry applications. |
| SwissProt ID | P51654 |
|---|
SDS-PAGE
SDS-PAGE for Rat Anti-Human GPC3 Antibody .
FC
Flow-cytometry using anti-human GPC3 antibody. Untransfected cells and Transfected cells were stained with an anti-human GPC3 monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Rat IgG H&L Polyclonal Antibody, FITC and cells analysed on a NovoCyte Flow Cytometer.
Bioactivity
Detects recombinant human Glypican-3/GPC3 in indirect ELISA.
Bioactivity
Detects recombinant mouse Glypican-3/GPC3 in indirect ELISA.
WB
Western blot analysis was performed using anti-GPC3 monoclonal antibody at 1ug/mL on various samples.Lane 1:recombinant human GPC3 Lane 2:negative control| Application Notes | ELISA:1:2000-1:20000 WB:1:500-1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. |
| Storage Buffer | 0.01M PBS, pH 7.4.Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
