SDS-PAGE
SDS-PAGE for InVivoMAb Anti-Mouse CD274/PD-L1/B7-H1 Antibody .FC
Flow-cytometry using FITC anti-Mouse PD1 antibody. Unstimulated cells and 3-day CoA-stimulated Mouse spleen cells were stained with an FITC anti-Mouse PD1 monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.Bioactivity
Detects Recombinant Mouse CD274/PD-L1/B7-H1 Protein in indirect ELISA.| Product Name | InVivoMAb Anti-Mouse PD-L1/B7-H1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2b |
| Clone Number | A1G3 |
| Host Species | Rat |
| Tested Applications | invivoPD-L1blockadeinvitroPD-L1blockadeIFIHC-FFCMWB |
| WB:1:200-1:2000 IHC:1:100-1:1000 |
|
| Species Reactivity | Mouse |
| Concentration | 1mg/ml |
| Purification | Protein A/G |
| Target | Programmed cell death 1 ligand 1, PD-L1, PDCD1 ligand 1, Programmed death ligand 1, B7 homolog 1, B7-H1, CD274, Cd274, B7h1, Pdcd1l1, Pdcd1lg1, Pdl1 |
|---|---|
| Function | InVivoMAb Anti-Mouse CD274/PD-L1/B7-H1 Antibody (10F.9G2)[10F.9G2] (MV974020) is arat monoclonalantibody detectingProgrammed cell death 1 ligand 1inin vivo PD-L1 blockade, in vitro PD-L1 blockade, IF, IHC-F, FCM, WB. Suitable forMouse.HighlightsMulti-Application— Validated across multiple applications.Flow Cytometry— Validated for flow cytometry applications. |
| SwissProt ID | Q9EP73 |
|---|
SDS-PAGE
SDS-PAGE for InVivoMAb Anti-Mouse CD274/PD-L1/B7-H1 Antibody .
FC
Flow-cytometry using FITC anti-Mouse PD1 antibody. Unstimulated cells and 3-day CoA-stimulated Mouse spleen cells were stained with an FITC anti-Mouse PD1 monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Bioactivity
Detects Recombinant Mouse CD274/PD-L1/B7-H1 Protein in indirect ELISA.| Application Notes | WB:1:200-1:2000 IHC:1:100-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. |
| Storage Buffer | 0.01M PBS, pH 7.4.Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
