WB
Western blot analysis was performed using anti-APOE4 monoclonal antibody at 1ug/mL on various samples.Lane 1:recombinant human APOE4 Lane 2:negative control
FC
Flow-cytometry using anti-human APOE antibody. HepG2 cells were stained with an irrelevant antibody or an anti-human APOE monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC and cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE
SDS-PAGE for InVivoMAb Anti-Human APOE2/3/4 Antibody