FC
Flow-cytometry using anti-human IL8 antibody.IL8 Transfected CHO cells were stained with an irrelevant antibody or an anti-human IL8 antibody monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody and cells analysed on a NovoCyte Flow Cytometer.SDS-PAGE
SDS-PAGE for InVivoMAb Anti-Human IL8/CXCL8 .WB
Western blot analysis was performed using anti-IL8/CXCL8 monoclonal antibody at 1ug/mL on various samples.Lane 1:recombinant human IL8/CXCL8 Lane 2:negative control| Product Name | InVivoMAb Anti-Human IL-8/CXCL8 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1,k |
| Clone Number | U2J1 |
| Host Species | Mouse |
| Tested Applications | ELISAFCMNeutralization |
| ELISA:1:2000-1:20000 |
|
| Species Reactivity | Human |
| Predicted Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A/G |
| Target | GCP/IL-8 protein II, MDNCF, IL8/NAP1 form III, IL-8(1-77), Neutrophil-activating factor, GCP/IL-8 protein I, GCP/IL-8 protein VI, Interleukin-8, Chemokine (C-X-C motif) ligand 8, Neutrophil-activating protein 1, Monocyte-derived neutrophil chemotactic factor, (Ser-IL-8)72, MDNCF-b, LYNAP, GCP/IL-8 protein V, IL8/NAP1 form IV, MONAP, IL8/NAP1 form V, IL8/NAP1 form VI, NAP-1, Granulocyte chemotactic protein 1, GCP/IL-8 protein III, IL8/NAP1 form II, IL8, IL-8, CXCL8, IL8/NAP1 form I, (Ala-IL-8)77, Protein 3-10C, GCP/IL-8 protein IV, Emoctakin, GCP-1, C-X-C motif chemokine 8, NAF, T-cell chemotactic factor, MDNCF-c, Monocyte-derived neutrophil-activating peptide, Lymphocyte-derived neutrophil-activating factor |
|---|---|
| Function | InVivoMAb Anti-Human IL8/CXCL8 (Iv0023)[Iv0023] (HY554010) is amouse monoclonalantibody detectingGCP/IL-8 protein IIinELISA, FCM, Neutralization. Suitable forHuman.Highlights●Multi-Application— Validated across multiple applications.●Flow Cytometry— Validated for flow cytometry applications. |
| SwissProt ID | P10145 |
|---|
FC
Flow-cytometry using anti-human IL8 antibody.IL8 Transfected CHO cells were stained with an irrelevant antibody or an anti-human IL8 antibody monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody and cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE
SDS-PAGE for InVivoMAb Anti-Human IL8/CXCL8 .
WB
Western blot analysis was performed using anti-IL8/CXCL8 monoclonal antibody at 1ug/mL on various samples.Lane 1:recombinant human IL8/CXCL8 Lane 2:negative control| Application Notes | ELISA:1:2000-1:20000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. |
| Storage Buffer | 0.01M PBS, pH 7.4.Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
