FC
Flow-cytometry using anti-human Galectin-9 antibody.Human Jurkat cell line were stained with an irrelevant antibody or an anti-human Galectin-9 antibody monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody and cells analysed on a NovoCyte Flow Cytometer.FC
Flow-cytometry using anti-human Galectin-9 antibody.MOLT-4 cells were stained with an irrelevant antibody or an anti-human Galectin-9 antibody monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody and cells analysed on a NovoCyte Flow Cytometer.FC
Flow-cytometry using anti-human Galectin-9 antibody.Human peripheral blood lymphocytes were stained with an irrelevant antibody or an anti-human Galectin-9 antibody monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody and cells analysed on a NovoCyte Flow Cytometer.SDS-PAGE
SDS-PAGE for Anti-Human Gal-9/LGALS9 Antibody .FC
Flow-cytometry using anti-human Galectin-9 antibody. THP-1 cells were fixed and permeabilized, then stained with an irrelevant antibody or an anti-human Galectin-9 monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC and cells analysed on a NovoCyte Flow Cytometer.| Product Name | Anti-Human Gal-9/LGALS9 Recombinant Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a,k |
| Clone Number | B4B7 |
| Host Species | Mouse |
| Tested Applications | BlockingELISAFCM |
| ELISA:1:2000-1:20000 |
|
| Species Reactivity | Human |
| Predicted Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A/G |
| Target | Ecalectin, LGALS9, Galectin-9, Gal-9, Tumor antigen HOM-HD-21 |
|---|---|
| Function | Anti-Human Gal-9/LGALS9 Antibody (G9.2-17)[G9.2-17] (HT173013) is amouse monoclonalantibody detectingEcalectininBlocking, ELISA, FCM. Suitable forHuman.HighlightsMulti-Application— Validated across multiple applications.Flow Cytometry— Validated for flow cytometry applications. |
| SwissProt ID | O00182 |
|---|
FC
Flow-cytometry using anti-human Galectin-9 antibody.Human Jurkat cell line were stained with an irrelevant antibody or an anti-human Galectin-9 antibody monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody and cells analysed on a NovoCyte Flow Cytometer.
FC
Flow-cytometry using anti-human Galectin-9 antibody.MOLT-4 cells were stained with an irrelevant antibody or an anti-human Galectin-9 antibody monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody and cells analysed on a NovoCyte Flow Cytometer.
FC
Flow-cytometry using anti-human Galectin-9 antibody.Human peripheral blood lymphocytes were stained with an irrelevant antibody or an anti-human Galectin-9 antibody monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody and cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE
SDS-PAGE for Anti-Human Gal-9/LGALS9 Antibody .
FC
Flow-cytometry using anti-human Galectin-9 antibody. THP-1 cells were fixed and permeabilized, then stained with an irrelevant antibody or an anti-human Galectin-9 monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC and cells analysed on a NovoCyte Flow Cytometer.| Application Notes | ELISA:1:2000-1:20000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. |
| Storage Buffer | 0.01M PBS, pH 7.4.Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
