Bioactivity
SEC-HPLC detection for Anti-Human B2M/Beta-2-microglobulin Antibody .Bioactivity
Detects B2M/Beta-2-microglobulin in indirect ELISAs.FC
Flow-cytometry using anti-human B2M antibody.HeLa cells were stained with an irrelevant antibody or an anti-human B2M antibody monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody and cells analysed on a NovoCyte Flow Cytometer.FC
Flow-cytometry using anti-human B2M antibody.Jurkat cells were stained with an irrelevant antibody or an anti-human B2M antibody monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody and cells analysed on a NovoCyte Flow Cytometer.FC
Flow-cytometry using anti-human B2M antibody.A431 cells were stained with an irrelevant antibody or an anti-human B2M antibody monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody and cells analysed on a NovoCyte Flow Cytometer.FC
Flow-cytometry using anti-human B2M antibody.Human peripheral blood lymphocytes were stained with an irrelevant antibody or an anti-human B2M antibody monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody and cells analysed on a NovoCyte Flow Cytometer.FC
Flow-cytometry using anti-human B2M antibody.Human peripheral blood lymphocytes were stained with an irrelevant antibody or an anti-human B2M antibody monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody and cells analysed on a NovoCyte Flow Cytometer.SDS-PAGE
SDS-PAGE for Anti-Human B2M/Beta-2-microglobulin Antibody .FC
Flow-cytometry using APC anti-human B2M antibody. Hela cells were stained with an irrelevant antibody or an APC anti-human B2M monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.FC
Flow-cytometry using FITC anti-human B2M antibody. Hela cells were stained with an irrelevant antibody or an FITC anti-human B2M monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.FC
Flow-cytometry using PE anti-human B2M antibody. Hela cells were stained with an irrelevant antibody or an PE anti-human B2M monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.WB
Western blot analysis was performed using anti-CD1a & B2M monoclonal antibody at 1ug/mL on various samples.Lane 1:recombinant human CD1a & B2M Lane 2:negative control| Product Name | Anti-Human B2M/Beta-2-microglobulin Recombinant Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2b,k |
| Clone Number | J7R4 |
| Host Species | Mouse |
| Tested Applications | ELISAFCMWB |
| ELISA:1:2000-1:20000 WB:1:500-1:2000 |
|
| Species Reactivity | Human |
| Predicted Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A/G |
| Target | Beta-2-microglobulin, Beta-2-microglobulin form pI 5.3, B2M, CDABP0092, HDCMA22P |
|---|---|
| Function | Anti-Human B2M/Beta-2-microglobulin Antibody (BBM.1)[BBM.1] (HX250013) is amouse monoclonalantibody detectingBeta-2-microglobulininELISA, FCM, WB. Suitable forHuman.HighlightsMulti-Application— Validated across multiple applications.Flow Cytometry— Validated for flow cytometry applications. |
| SwissProt ID | P61769 |
|---|
Bioactivity
SEC-HPLC detection for Anti-Human B2M/Beta-2-microglobulin Antibody .
Bioactivity
Detects B2M/Beta-2-microglobulin in indirect ELISAs.
FC
Flow-cytometry using anti-human B2M antibody.HeLa cells were stained with an irrelevant antibody or an anti-human B2M antibody monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody and cells analysed on a NovoCyte Flow Cytometer.
FC
Flow-cytometry using anti-human B2M antibody.Jurkat cells were stained with an irrelevant antibody or an anti-human B2M antibody monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody and cells analysed on a NovoCyte Flow Cytometer.
FC
Flow-cytometry using anti-human B2M antibody.A431 cells were stained with an irrelevant antibody or an anti-human B2M antibody monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody and cells analysed on a NovoCyte Flow Cytometer.
FC
Flow-cytometry using anti-human B2M antibody.Human peripheral blood lymphocytes were stained with an irrelevant antibody or an anti-human B2M antibody monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody and cells analysed on a NovoCyte Flow Cytometer.
FC
Flow-cytometry using anti-human B2M antibody.Human peripheral blood lymphocytes were stained with an irrelevant antibody or an anti-human B2M antibody monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody and cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE
SDS-PAGE for Anti-Human B2M/Beta-2-microglobulin Antibody .
FC
Flow-cytometry using APC anti-human B2M antibody. Hela cells were stained with an irrelevant antibody or an APC anti-human B2M monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.
FC
Flow-cytometry using FITC anti-human B2M antibody. Hela cells were stained with an irrelevant antibody or an FITC anti-human B2M monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.
FC
Flow-cytometry using PE anti-human B2M antibody. Hela cells were stained with an irrelevant antibody or an PE anti-human B2M monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.
WB
Western blot analysis was performed using anti-CD1a & B2M monoclonal antibody at 1ug/mL on various samples.Lane 1:recombinant human CD1a & B2M Lane 2:negative control| Application Notes | ELISA:1:2000-1:20000 WB:1:500-1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. |
| Storage Buffer | 0.01M PBS, pH 7.4.Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
