FC
Flow-cytometry using anti-human TSHR antibody.TSHR Transfected CHO cells were stained with an irrelevant antibody or an anti-human TSHR antibody monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody and cells analysed on a NovoCyte Flow Cytometer.SDS-PAGE
SDS-PAGE for Anti-Human TSHR Antibody .WB
Western blot analysis was performed using anti-TSHR monoclonal antibody at 1ug/mL on various samples.Lane 1:Mouse ovary lysate| Product Name | Anti-Human TSHR Recombinant Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a,lambda |
| Clone Number | A9D6 |
| Host Species | Mouse |
| Tested Applications | ELISAFCMIHCWB |
| ELISA:1:2000-1:20000 WB:1:500-1:2000 IHC:1:100-1:1000 |
|
| Species Reactivity | Human |
| Predicted Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A/G |
| Target | Thyroid-stimulating hormone receptor, TSH-R, TSHR, Thyrotropin receptor, LGR3 |
|---|---|
| Function | Anti-Human TSHR Antibody (SAA0401)[SAA0401] (HB852013) is amouse monoclonalantibody detectingThyroid-stimulating hormone receptorinELISA, FCM, IHC, WB. Suitable forHuman.HighlightsMulti-Application— Validated across multiple applications.Flow Cytometry— Validated for flow cytometry applications. |
| SwissProt ID | P16473 |
|---|
FC
Flow-cytometry using anti-human TSHR antibody.TSHR Transfected CHO cells were stained with an irrelevant antibody or an anti-human TSHR antibody monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody and cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE
SDS-PAGE for Anti-Human TSHR Antibody .
WB
Western blot analysis was performed using anti-TSHR monoclonal antibody at 1ug/mL on various samples.Lane 1:Mouse ovary lysate| Application Notes | ELISA:1:2000-1:20000 WB:1:500-1:2000 IHC:1:100-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. |
| Storage Buffer | 0.01M PBS, pH 7.4.Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
