SDS-PAGE
SDS-PAGE for Anti-Human GAPDH Monoclonal AntibodyWB
Western blot analysis was performed using anti-GAPDH monoclonal antibody at 1ug/mL on various samples.Lane 1:MCF-7 cell lysateLane 2:HepG2 cell lysateLane 3:NIH3T3 cell lysateLane 4:PC-12 cell lysateLane 5:PC-3 cell lysateLane 6:Mouse kidney lysateLane 7:Rat spleen lysateLane 8:Rat kidney lysate| Product Name | Anti-Human GAPDH Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E. coli - derived recombinant Human GAPDH protein (Met1-Glu335). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2b |
| Clone Number | Q4W6 |
| Host Species | Mouse |
| Tested Applications | ELISAWB |
| ELISA:1:1000-1:10000 WB:1:200-1:2000 |
|
| Species Reactivity | Human Mouse Rat |
| Predicted Reactivity | Human, Mouse, Rat |
| Concentration | 1mg/ml |
| Purification | Protein A/G |
| Target | GAPD, Peptidyl-cysteine S-nitrosylase GAPDH, GAPDH, Glyceraldehyde-3-phosphate dehydrogenase |
|---|---|
| Function | Anti-Human GAPDH Monoclonal Antibody (1A200)[1A200] (HY030035) is amouse monoclonalantibody detectingGAPDHinELISA, WB. Suitable forHuman, Mouse, and Rat.Highlights●Recombinant— Superior batch-to-batch reproducibility with defined sequences.●Multi-Species— Cross-reactive for translational research. |
SDS-PAGE
SDS-PAGE for Anti-Human GAPDH Monoclonal Antibody
WB
Western blot analysis was performed using anti-GAPDH monoclonal antibody at 1ug/mL on various samples.Lane 1:MCF-7 cell lysateLane 2:HepG2 cell lysateLane 3:NIH3T3 cell lysateLane 4:PC-12 cell lysateLane 5:PC-3 cell lysateLane 6:Mouse kidney lysateLane 7:Rat spleen lysateLane 8:Rat kidney lysate| Application Notes | ELISA:1:1000-1:10000 WB:1:200-1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. |
| Storage Buffer | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
