FC
Flow-cytometry using FITC anti-Mouse CD20 antibody. Mouse spleen cells were stained with an irrelevant antibody or an FITC conjugated anti-Mouse CD20 monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing,cells analysed on a NovoCyte Flow Cytometer.
FC
Flow-cytometry using anti-Mouse CD20 antibody. Untransfected cells and Transfected cells were stained with an anti-Mouse CD20 monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC and cells analysed on a NovoCyte Flow Cytometer.