FC
Flow-cytometry using anti-scFv antibody. Transfected cells were stained with an irrelevant antibody or an anti-scFv monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC and cells analysed on a NovoCyte Flow Cytometer.