WB
Western blot analysis was performed using anti-CD86/B7-2 monoclonal antibody at 1ug/mL on various samples.Lane 1:recombinant human CD86/B7-2 Lane 2:negative controlFC
Flow-cytometry using anti-human CD86 antibody. Daudi cells were stained with an irrelevant antibody or an anti-human CD86 monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC and cells analysed on a NovoCyte Flow Cytometer.| Product Name | Anti-Human CD86/B7-2 Recombinant Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1,k |
| Clone Number | Z7O1 |
| Host Species | Mouse |
| Tested Applications | ELISAFCMIP |
| ELISA:1:2000-1:20000 IP:1:10-1:100 |
|
| Species Reactivity | Human Pig |
| Predicted Reactivity | Human, Pig |
| Concentration | 1mg/ml |
| Purification | Protein A/G |
| Target | T-lymphocyte activation antigen CD86, Activation B7-2 antigen, B70, BU63, CTLA-4 counter-receptor B7.2, FUN-1, CD86, CD86, CD28LG2, B7-2 |
|---|---|
| Function | Anti-Human CD86/B7-2 Antibody (3D1)[3D1] (HW776023) is amouse monoclonalantibody detectingT-lymphocyte activation antigen CD86inELISA, FCM, IP. Suitable forHuman and Pig.HighlightsMulti-Application— Validated across multiple applications.Flow Cytometry— Validated for flow cytometry applications. |
WB
Western blot analysis was performed using anti-CD86/B7-2 monoclonal antibody at 1ug/mL on various samples.Lane 1:recombinant human CD86/B7-2 Lane 2:negative control
FC
Flow-cytometry using anti-human CD86 antibody. Daudi cells were stained with an irrelevant antibody or an anti-human CD86 monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC and cells analysed on a NovoCyte Flow Cytometer.| Application Notes | ELISA:1:2000-1:20000 IP:1:10-1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Storage Buffer | 0.01M PBS pH 7.4Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
