FC
Flow-cytometry using anti-human DNMT3A antibody.293T cells were stained with an irrelevant antibody or an anti-human DNMT3A antibody monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody and cells analysed on a NovoCyte Flow Cytometer.FC
Flow-cytometry using anti-human DNMT3A antibody.HepG2 cells were stained with an irrelevant antibody or an anti-human DNMT3A antibody monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody and cells analysed on a NovoCyte Flow Cytometer.FC
Flow-cytometry using anti-human DNMT3A antibody.Human Jurkat cell line were stained with an irrelevant antibody or an anti-human DNMT3A antibody monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody and cells analysed on a NovoCyte Flow Cytometer.FC
Flow-cytometry using anti-human DNMT3A antibody.HeLa cells were stained with an irrelevant antibody or an anti-human DNMT3A antibody monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody and cells analysed on a NovoCyte Flow Cytometer.SDS-PAGE
SDS-PAGE for Anti-Human DNMT3A IP Antibody .FC
Flow-cytometry using FITC anti-human DNMT3A antibody. HepG-2 cells were fixed and permeabilized, then stained with an irrelevant antibody or an FITC anti-human DNMT3A monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.FC
Flow-cytometry using PE anti-human DNMT3A antibody. HepG-2 cells were fixed and permeabilized, then stained with an irrelevant antibody or an PE anti-human DNMT3A monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.| Product Name | Anti-Human DNMT3A IP Recombinant Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | S5B7 |
| Host Species | Mouse |
| Tested Applications | ELISAFCMIFIP |
| ELISA:1:2000-1:20000 IP:1:10-1:100 |
|
| Species Reactivity | Human |
| Predicted Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A/G |
| Target | Dnmt3a, DNA MTase HsaIIIA, DNMT3A, M.HsaIIIA, DNA (cytosine-5)-methyltransferase 3A, DNA methyltransferase HsaIIIA |
|---|---|
| Function | Anti-Human DNMT3A IP Antibody (SAA0199)[SAA0199] (HV233013) is amouse monoclonalantibody detectingDnmt3ainELISA, FCM, IF, IP. Suitable forHuman.HighlightsMulti-Application— Validated across multiple applications.Flow Cytometry— Validated for flow cytometry applications. |
| SwissProt ID | Q9Y6K1 |
|---|
FC
Flow-cytometry using anti-human DNMT3A antibody.293T cells were stained with an irrelevant antibody or an anti-human DNMT3A antibody monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody and cells analysed on a NovoCyte Flow Cytometer.
FC
Flow-cytometry using anti-human DNMT3A antibody.HepG2 cells were stained with an irrelevant antibody or an anti-human DNMT3A antibody monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody and cells analysed on a NovoCyte Flow Cytometer.
FC
Flow-cytometry using anti-human DNMT3A antibody.Human Jurkat cell line were stained with an irrelevant antibody or an anti-human DNMT3A antibody monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody and cells analysed on a NovoCyte Flow Cytometer.
FC
Flow-cytometry using anti-human DNMT3A antibody.HeLa cells were stained with an irrelevant antibody or an anti-human DNMT3A antibody monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody and cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE
SDS-PAGE for Anti-Human DNMT3A IP Antibody .
FC
Flow-cytometry using FITC anti-human DNMT3A antibody. HepG-2 cells were fixed and permeabilized, then stained with an irrelevant antibody or an FITC anti-human DNMT3A monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
FC
Flow-cytometry using PE anti-human DNMT3A antibody. HepG-2 cells were fixed and permeabilized, then stained with an irrelevant antibody or an PE anti-human DNMT3A monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.| Application Notes | ELISA:1:2000-1:20000 IP:1:10-1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. |
| Storage Buffer | 0.01M PBS, pH 7.4.Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
