FC
Flow-cytometry using anti-human CD31 antibody. Jurkat cells were stained with an irrelevant antibody or an anti-human CD31 monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC and cells analysed on a NovoCyte Flow Cytometer.FC
Flow-cytometry using anti-human CD31 antibody. THP-1 cells were stained with an irrelevant antibody or an anti-human CD31 antibody monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody and cells analysed on a NovoCyte Flow Cytometer.SDS-PAGE
SDS-PAGE for Anti-Human CD31/PECAM1 Antibody| Product Name | Anti-Human CD31/PECAM1 Recombinant Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Isotype | VHH,mFc |
| Clone Number | M9M3 |
| Host Species | Mouse |
| Tested Applications | ELISAWBFCM |
| ELISA:1:2000-1:20000 WB:1:500-1:2000 |
|
| Species Reactivity | Human |
| Predicted Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A/G |
| Target | Platelet endothelial cell adhesion molecule, PECAM-1, EndoCAM, GPIIA', PECA1, CD31, PECAM1 |
|---|---|
| Function | Anti-Human CD31/PECAM1 Antibody (SAA2178)[SAA2178] (HB004023) is amouse monoclonalantibody detectingPECAM1inELISA, WB, FCM. Suitable forHuman.Highlights●Multi-Application— Validated across multiple applications.●Flow Cytometry— Validated for flow cytometry applications. |
| SwissProt ID | P16284 |
|---|
FC
Flow-cytometry using anti-human CD31 antibody. Jurkat cells were stained with an irrelevant antibody or an anti-human CD31 monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC and cells analysed on a NovoCyte Flow Cytometer.
FC
Flow-cytometry using anti-human CD31 antibody. THP-1 cells were stained with an irrelevant antibody or an anti-human CD31 antibody monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody and cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE
SDS-PAGE for Anti-Human CD31/PECAM1 Antibody| Application Notes | ELISA:1:2000-1:20000 WB:1:500-1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. |
| Storage Buffer | 0.01M PBS, pH 7.4.Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
