Bioactivity
Detects Human LGALS3 in indirect ELISAs.FC
Flow-cytometry using anti-human Galectin-3 antibody.HaCaT cells were stained with an irrelevant antibody or an anti-human Galectin-3 antibody monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody and cells analysed on a NovoCyte Flow Cytometer.FC
Flow-cytometry using anti-human Galectin-3 antibody.MCF7 cells were stained with an irrelevant antibody or an anti-human Galectin-3 antibody monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody and cells analysed on a NovoCyte Flow Cytometer.FC
Flow-cytometry using anti-human Galectin-3 antibody.HeLa cells were stained with an irrelevant antibody or an anti-human Galectin-3 antibody monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody and cells analysed on a NovoCyte Flow Cytometer.FC
Flow-cytometry using anti-human Galectin-3 antibody.SiHa cells were stained with an irrelevant antibody or an anti-human Galectin-3 antibody monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody and cells analysed on a NovoCyte Flow Cytometer.SDS-PAGE
SDS-PAGE for Anti-Human Gal3/Galectin-3 Antibody .FC
Flow-cytometry using anti-human Galectin-3 antibody. 5637 cells were fixed and permeabilized, then stained with an irrelevant antibody or an anti-human Galectin-3 monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC and cells analysed on a NovoCyte Flow Cytometer.| Product Name | Anti-Human Gal3/Galectin-3 Recombinant Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a,k |
| Clone Number | N6Y4 |
| Host Species | Mouse |
| Tested Applications | ELISAFCM |
| ELISA:1:2000-1:20000 |
|
| Species Reactivity | Human |
| Predicted Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A/G |
| Target | Mac-2 antigen, Lectin L-29, LGALS3, Galactose-specific lectin 3, 35 kDa lectin, Galactoside-binding protein, CBP 35, L-31, Gal-3, Laminin-binding protein, Galectin-3, GALBP, IgE-binding protein, Carbohydrate-binding protein 35, MAC2 |
|---|---|
| Function | Anti-Human Gal3/Galectin-3 Antibody (M3/38)[M3/38] (HB167023) is amouse monoclonalantibody detectingMac-2 antigeninELISA, FCM. Suitable forHuman.HighlightsFlow Cytometry— Validated for flow cytometry applications. |
| SwissProt ID | P17931 |
|---|
Bioactivity
Detects Human LGALS3 in indirect ELISAs.
FC
Flow-cytometry using anti-human Galectin-3 antibody.HaCaT cells were stained with an irrelevant antibody or an anti-human Galectin-3 antibody monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody and cells analysed on a NovoCyte Flow Cytometer.
FC
Flow-cytometry using anti-human Galectin-3 antibody.MCF7 cells were stained with an irrelevant antibody or an anti-human Galectin-3 antibody monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody and cells analysed on a NovoCyte Flow Cytometer.
FC
Flow-cytometry using anti-human Galectin-3 antibody.HeLa cells were stained with an irrelevant antibody or an anti-human Galectin-3 antibody monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody and cells analysed on a NovoCyte Flow Cytometer.
FC
Flow-cytometry using anti-human Galectin-3 antibody.SiHa cells were stained with an irrelevant antibody or an anti-human Galectin-3 antibody monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody and cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE
SDS-PAGE for Anti-Human Gal3/Galectin-3 Antibody .
FC
Flow-cytometry using anti-human Galectin-3 antibody. 5637 cells were fixed and permeabilized, then stained with an irrelevant antibody or an anti-human Galectin-3 monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC and cells analysed on a NovoCyte Flow Cytometer.| Application Notes | ELISA:1:2000-1:20000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. |
| Storage Buffer | 0.01M PBS, pH 7.4.Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
