SDS-PAGE
SDS-PAGE for Anti-Human CTSB Antibody .
FC
Flow-cytometry using FITC anti-human CTSB antibody. HepG-2 cells were fixed and permeabilized, then stained with an irrelevant antibody or an FITC anti-human CTSB monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
WB
Western blot analysis was performed using anti-CTSB monoclonal antibody at 1μg/ml on various samples.Lane 1:recombinant human CTSB , 50ngLane 2:recombinant human CTSB , 30ngLane 3:recombinant human CTSB , 20ng