SDS-PAGE
SDS-PAGE for Anti-Human HRAS Antibody .FC
Flow-cytometry using FITC anti-human HRAS antibody. MCF-7 cells were fixed and permeabilized, then stained with an irrelevant antibody or an FITC anti-human HRAS monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.WB
Western blot analysis was performed using anti-KRAS/K-Ras 2 monoclonal antibody at 1μg/ml on various samples.Lane 1:recombinant human KRAS/K-Ras 2 , 50 ngLane 2:recombinant human KRAS/K-Ras 2 , 30 ng| Product Name | Anti-Human HRAS Recombinant Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a,k |
| Clone Number | B5Q9 |
| Host Species | Mouse |
| Tested Applications | IFIPWB |
| WB:1:500-1:2000 IP:1:10-1:100 |
|
| Species Reactivity | Human |
| Predicted Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A/G |
| Target | c-H-ras, GTPase HRas, H-Ras-1, p21ras, Transforming protein p21, Ha-Ras, HRAS1, HRAS |
|---|---|
| Function | Anti-Human HRAS Antibody (Y13-259)[Y13-259] (HF778023) is amouse monoclonalantibody detectingc-H-rasinIF, IP, WB. Suitable forHuman.HighlightsMulti-Application— Validated across multiple applications. |
| SwissProt ID | P01112 |
|---|
SDS-PAGE
SDS-PAGE for Anti-Human HRAS Antibody .
FC
Flow-cytometry using FITC anti-human HRAS antibody. MCF-7 cells were fixed and permeabilized, then stained with an irrelevant antibody or an FITC anti-human HRAS monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.
WB
Western blot analysis was performed using anti-KRAS/K-Ras 2 monoclonal antibody at 1μg/ml on various samples.Lane 1:recombinant human KRAS/K-Ras 2 , 50 ngLane 2:recombinant human KRAS/K-Ras 2 , 30 ng| Application Notes | WB:1:500-1:2000 IP:1:10-1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. |
| Storage Buffer | 0.01M PBS, pH 7.4.Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
