FC
Flow-cytometry using anti-human MMP7 antibody.A549 cells were stained with an irrelevant antibody or an anti-human MMP7 antibody monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody and cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE
SDS-PAGE for Anti-Human MMP7 Antibody .
WB
Western blot analysis was performed using anti-MMP7 monoclonal antibody at 1μg/ml on various samples.Lane 1:recombinant human MMP7 , 50ngLane 2:recombinant human MMP7 , 30ngLane 3:recombinant human MMP7 , 20ngLane 4:recombinant human MMP7 , 10ng