FC
Flow-cytometry using PE anti-human BCL2 antibody. A549 cells were fixed and permeabilized, then stained with an irrelevant antibody or an PE anti-human BCL2 monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.FC
Flow-cytometry using FITC anti-human BCL2 antibody. A549 cells were fixed and permeabilized, then stained with an irrelevant antibody or an FITC anti-human BCL2 monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.| Product Name | Anti-Human Bcl2 Recombinant Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Isotype | VHH,mFc |
| Clone Number | T9H7 |
| Host Species | Mouse |
| Tested Applications | ELISAFCM |
| ELISA:1:2000-1:20000 |
|
| Species Reactivity | Human |
| Predicted Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A/G |
| Target | Apoptosis regulator Bcl-2, BCL2 |
|---|---|
| Function | Anti-Human Bcl2 Antibody (SAA1989)[SAA1989] (HY131023) is amouse monoclonalantibody detectingApoptosis regulator Bcl-2inELISA, FCM. Suitable forHuman.HighlightsFlow Cytometry— Validated for flow cytometry applications. |
| SwissProt ID | P10415 |
|---|
FC
Flow-cytometry using PE anti-human BCL2 antibody. A549 cells were fixed and permeabilized, then stained with an irrelevant antibody or an PE anti-human BCL2 monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
FC
Flow-cytometry using FITC anti-human BCL2 antibody. A549 cells were fixed and permeabilized, then stained with an irrelevant antibody or an FITC anti-human BCL2 monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.| Application Notes | ELISA:1:2000-1:20000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. |
| Storage Buffer | 0.01M PBS, pH 7.4.Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
