Bioactivity
Detects Rat VEGFA in indirect ELISAs.SDS-PAGE
SDS-PAGE for Anti-Rat VEGFA Antibody .FC
Flow-cytometry using FITC anti-human VEGF antibody. U87 cells were fixed and permeabilized, then stained with an irrelevant antibody or an FITC anti-human VEGF monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.FC
Flow-cytometry using PE anti-human VEGF antibody. U87 cells were fixed and permeabilized, then stained with an irrelevant antibody or an PE anti-human VEGF monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.WB
Western blot analysis was performed using anti-VEGFA/VEGF165 monoclonal antibody at 1ug/mL on various samples.Lane 1:recombinant human VEGFA/VEGF165 Lane 2:negative control| Product Name | Anti-Rat VEGFA Recombinant Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a,k |
| Clone Number | H4Z4 |
| Host Species | Mouse |
| Tested Applications | BlockingELISA |
| ELISA:1:2000-1:20000 |
|
| Species Reactivity | Human |
| Predicted Reactivity | Human, Mouse, Rat, Rabbit |
| Concentration | 1mg/ml |
| Purification | Protein A/G |
| Target | Vascular endothelial growth factor A, VPF, VEGFA, VEGF, Vascular permeability factor, VEGF-A |
|---|---|
| Function | Anti-Rat VEGFA Antibody (B20-4)[B20-4] (RB941013) is amouse monoclonalantibody detectingVascular endothelial growth factor AinBlocking, ELISA. Suitable forHuman, Mouse, Rat, and Rabbit.HighlightsMulti-Species— Cross-reactive for translational research. |
| SwissProt ID | P16612 |
|---|
Bioactivity
Detects Rat VEGFA in indirect ELISAs.
SDS-PAGE
SDS-PAGE for Anti-Rat VEGFA Antibody .
FC
Flow-cytometry using FITC anti-human VEGF antibody. U87 cells were fixed and permeabilized, then stained with an irrelevant antibody or an FITC anti-human VEGF monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
FC
Flow-cytometry using PE anti-human VEGF antibody. U87 cells were fixed and permeabilized, then stained with an irrelevant antibody or an PE anti-human VEGF monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
WB
Western blot analysis was performed using anti-VEGFA/VEGF165 monoclonal antibody at 1ug/mL on various samples.Lane 1:recombinant human VEGFA/VEGF165 Lane 2:negative control| Application Notes | ELISA:1:2000-1:20000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. |
| Storage Buffer | 0.01M PBS, pH 7.4.Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
