SDS-PAGE
SDS-PAGE for Anti-Human FABP4/aP2 Antibody .
FC
Flow-cytometry using PE anti-human FABP4 antibody. Hela cells were fixed and permeabilized, then stained with an irrelevant antibody or an PE anti-human FABP4 monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
WB
Western blot analysis was performed using anti-FABP4 monoclonal antibody at 1µg/ml on various samples.Lane 1:recombinant human FABP4 Lane 2:negative control