Bioactivity
SEC-HPLC detection for Anti-Human BAX Antibody .FC
Flow-cytometry using anti-human BAX antibody.HepG2 cells were stained with an irrelevant antibody or an anti-human BAX antibody monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody and cells analysed on a NovoCyte Flow Cytometer.FC
Flow-cytometry using anti-human BAX antibody.Human Jurkat cell line were stained with an irrelevant antibody or an anti-human BAX antibody monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody and cells analysed on a NovoCyte Flow Cytometer.FC
Flow-cytometry using anti-human BAX antibody.A549 cells were stained with an irrelevant antibody or an anti-human BAX antibody monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody and cells analysed on a NovoCyte Flow Cytometer.SDS-PAGE
SDS-PAGE for Anti-Human BAX Antibody .FC
Flow-cytometry using FITC anti-human BAX antibody. TNFa stimulated U937 cells were fixed and permeabilized, then stained with an irrelevant antibody or an FITC anti-human BAX monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.FC
Flow-cytometry using PE anti-human BAX antibody. TNFa stimulated U937 cells were fixed and permeabilized, then stained with an irrelevant antibody or an PE anti-human BAX monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.| Product Name | Anti-Human BAX Recombinant Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1,k |
| Clone Number | L6B5 |
| Host Species | Mouse |
| Tested Applications | ELISAFCMWB |
| ELISA:1:2000-1:20000 WB:1:500-1:2000 |
|
| Species Reactivity | Human Mouse |
| Predicted Reactivity | Human, Mouse |
| Concentration | 1mg/ml |
| Purification | Protein A/G |
| Target | BAX, Bcl2-L-4, BCL2L4, Bcl-2-like protein 4, Apoptosis regulator BAX |
|---|---|
| Function | Anti-Human BAX Antibody (6A7)[6A7] (HC491013) is amouse monoclonalantibody detectingBAXinELISA, FCM, WB. Suitable forHuman and Mouse.HighlightsMulti-Application— Validated across multiple applications.Flow Cytometry— Validated for flow cytometry applications. |
| SwissProt ID | Q07812 |
|---|
Bioactivity
SEC-HPLC detection for Anti-Human BAX Antibody .
FC
Flow-cytometry using anti-human BAX antibody.HepG2 cells were stained with an irrelevant antibody or an anti-human BAX antibody monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody and cells analysed on a NovoCyte Flow Cytometer.
FC
Flow-cytometry using anti-human BAX antibody.Human Jurkat cell line were stained with an irrelevant antibody or an anti-human BAX antibody monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody and cells analysed on a NovoCyte Flow Cytometer.
FC
Flow-cytometry using anti-human BAX antibody.A549 cells were stained with an irrelevant antibody or an anti-human BAX antibody monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody and cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE
SDS-PAGE for Anti-Human BAX Antibody .
FC
Flow-cytometry using FITC anti-human BAX antibody. TNFa stimulated U937 cells were fixed and permeabilized, then stained with an irrelevant antibody or an FITC anti-human BAX monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.
FC
Flow-cytometry using PE anti-human BAX antibody. TNFa stimulated U937 cells were fixed and permeabilized, then stained with an irrelevant antibody or an PE anti-human BAX monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.| Application Notes | ELISA:1:2000-1:20000 WB:1:500-1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. |
| Storage Buffer | 0.01M PBS, pH 7.4.Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
