FC
Flow-cytometry using anti-human HIF1A antibody.HeLa cells were stained with an irrelevant antibody or an anti-human HIF1A antibody monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody and cells analysed on a NovoCyte Flow Cytometer.| Product Name | Anti-Human HIF1A Recombinant Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Isotype | VHH,mFc |
| Clone Number | R2I5 |
| Host Species | Mouse |
| Tested Applications | ELISAFCMIPWB |
| ELISA:1:2000-1:20000 WB:1:500-1:2000 IP:1:10-1:100 |
|
| Species Reactivity | Human |
| Predicted Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A/G |
| Target | HIF1A, MOP1, bHLHe78, Basic-helix-loop-helix-PAS protein MOP1, HIF-1-alpha, BHLHE78, Member of PAS protein 1, Hypoxia-inducible factor 1-alpha, ARNT-interacting protein, PAS domain-containing protein 8, HIF1-alpha, PASD8, Class E basic helix-loop-helix protein 78 |
|---|---|
| Function | Anti-Human HIF1A Antibody (SAA0814)[SAA0814] (HS858013) is amouse monoclonalantibody detectingHIF1AinELISA, FCM, IP, WB. Suitable forHuman.HighlightsMulti-Application— Validated across multiple applications.Flow Cytometry— Validated for flow cytometry applications. |
| SwissProt ID | Q16665 |
|---|
FC
Flow-cytometry using anti-human HIF1A antibody.HeLa cells were stained with an irrelevant antibody or an anti-human HIF1A antibody monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody and cells analysed on a NovoCyte Flow Cytometer.| Application Notes | ELISA:1:2000-1:20000 WB:1:500-1:2000 IP:1:10-1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. |
| Storage Buffer | 0.01M PBS, pH 7.4.Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
