Bioactivity
Detects Human ARG1/Arginase-1 in indirect ELISAs.FC
Flow-cytometry using anti-human Arginase 1 antibody.M2 macrophages derived from THP-1 cells were stained with an irrelevant antibody or an anti-human Arginase 1 antibody monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody and cells analysed on a NovoCyte Flow Cytometer.FC
Flow-cytometry using anti-human Arginase 1 antibody.Human peripheral blood lymphocytes were stained with an irrelevant antibody or an anti-human Arginase 1 antibody monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody and cells analysed on a NovoCyte Flow Cytometer.SDS-PAGE
SDS-PAGE for InVivoMAb Anti-Human ARG1/Arginase-1 Antibody .FC
Flow-cytometry using PE anti-human Arginase 1 antibody. HepG-2 cells were fixed and permeabilized, then stained with an irrelevant antibody or an PE anti-human Arginase 1 monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.| Product Name | InVivoMAb Anti-Human ARG1/Arginase-1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a,k |
| Clone Number | I8B6 |
| Host Species | Mouse |
| Tested Applications | ELISAFCM |
| ELISA:1:1000-1:10000 |
|
| Species Reactivity | Human |
| Predicted Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A/G |
| Target | Liver-type arginase, ARG1, Type I arginase, Arginase-1 |
|---|---|
| Function | InVivoMAb Anti-Human ARG1/Arginase-1 Antibody (Iv0120)[Iv0120] (HY339010) is amouse monoclonalantibody detectingLiver-type arginaseinELISA, FCM, Neutralization. Suitable forHuman.HighlightsMulti-Application— Validated across multiple applications.Flow Cytometry— Validated for flow cytometry applications. |
| SwissProt ID | P05089 |
|---|
Bioactivity
Detects Human ARG1/Arginase-1 in indirect ELISAs.
FC
Flow-cytometry using anti-human Arginase 1 antibody.M2 macrophages derived from THP-1 cells were stained with an irrelevant antibody or an anti-human Arginase 1 antibody monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody and cells analysed on a NovoCyte Flow Cytometer.
FC
Flow-cytometry using anti-human Arginase 1 antibody.Human peripheral blood lymphocytes were stained with an irrelevant antibody or an anti-human Arginase 1 antibody monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody and cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE
SDS-PAGE for InVivoMAb Anti-Human ARG1/Arginase-1 Antibody .
FC
Flow-cytometry using PE anti-human Arginase 1 antibody. HepG-2 cells were fixed and permeabilized, then stained with an irrelevant antibody or an PE anti-human Arginase 1 monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.| Application Notes | ELISA:1:1000-1:10000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. |
| Storage Buffer | 0.01M PBS, pH 7.4.Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
