SDS-PAGE
SDS-PAGE for Anti-Human FN1/Fibronectin AntibodyFC
Flow-cytometry using anti-human FN1 antibody. HepG2 cells were stained with an irrelevant antibody or an anti-human FN1 monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC and cells analysed on a NovoCyte Flow Cytometer.| Product Name | Anti-Human FN1/Fibronectin Recombinant Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a,k |
| Clone Number | X6M4 |
| Host Species | Mouse |
| Tested Applications | ELISAIFIHCSPRWB |
| ELISA:1:2000-1:20000 WB:1:500-1:2000 IHC:1:100-1:1000 |
|
| Species Reactivity | Human Mouse Rat |
| Predicted Reactivity | Human, Mouse, Rat |
| Concentration | 1mg/ml |
| Purification | Protein A/G |
| Target | Cold-insoluble globulin, CIG, FN, Fibronectin, FN1 |
|---|---|
| Function | Anti-Human FN1/Fibronectin Antibody (SAA0522)[SAA0522] (HY259013) is amouse monoclonalantibody detectingCold-insoluble globulininELISA, IF, IHC, SPR, WB. Suitable forHuman, Mouse, and Rat.HighlightsMulti-Application— Validated across multiple applications.Multi-Species— Cross-reactive for translational research. |
| SwissProt ID | P02751 |
|---|
SDS-PAGE
SDS-PAGE for Anti-Human FN1/Fibronectin Antibody
FC
Flow-cytometry using anti-human FN1 antibody. HepG2 cells were stained with an irrelevant antibody or an anti-human FN1 monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC and cells analysed on a NovoCyte Flow Cytometer.| Application Notes | ELISA:1:2000-1:20000 WB:1:500-1:2000 IHC:1:100-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. |
| Storage Buffer | 0.01M PBS, pH 7.4.Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
