FC
Flow-cytometry using anti-human CFH antibody.HepG2 cells were stained with an irrelevant antibody or an anti-human CFH antibody monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody and cells analysed on a NovoCyte Flow Cytometer.SDS-PAGE
SDS-PAGE for Anti-Human CFH Antibody .FC
Flow-cytometry using PE anti-human CFH antibody. HepG-2 cells were fixed and permeabilized, then stained with an irrelevant antibody or an PE anti-human CFH monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.FC
Flow-cytometry using FITC anti-human CFH antibody. HepG-2 cells were fixed and permeabilized, then stained with an irrelevant antibody or an FITC anti-human CFH monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.FC
Flow-cytometry using APC anti-human CFH antibody. HepG-2 cells were fixed and permeabilized, then stained with an irrelevant antibody or an APC anti-human CFH monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.| Product Name | Anti-Human CFH Recombinant Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a,k |
| Clone Number | H1I9 |
| Host Species | Mouse |
| Tested Applications | ELISAFCMWB |
| ELISA:1:2000-1:20000 WB:1:500-1:2000 |
|
| Species Reactivity | Human |
| Predicted Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A/G |
| Target | Complement factor H, CFH, H factor 1, HF1, HF, HF2 |
|---|---|
| Function | Anti-Human CFH Antibody (SAA0527)[SAA0527] (HY505013) is amouse monoclonalantibody detectingComplement factor HinELISA, FCM, WB. Suitable forHuman.HighlightsMulti-Application— Validated across multiple applications.Flow Cytometry— Validated for flow cytometry applications. |
| SwissProt ID | P08603 |
|---|
FC
Flow-cytometry using anti-human CFH antibody.HepG2 cells were stained with an irrelevant antibody or an anti-human CFH antibody monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody and cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE
SDS-PAGE for Anti-Human CFH Antibody .
FC
Flow-cytometry using PE anti-human CFH antibody. HepG-2 cells were fixed and permeabilized, then stained with an irrelevant antibody or an PE anti-human CFH monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
FC
Flow-cytometry using FITC anti-human CFH antibody. HepG-2 cells were fixed and permeabilized, then stained with an irrelevant antibody or an FITC anti-human CFH monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
FC
Flow-cytometry using APC anti-human CFH antibody. HepG-2 cells were fixed and permeabilized, then stained with an irrelevant antibody or an APC anti-human CFH monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.| Application Notes | ELISA:1:2000-1:20000 WB:1:500-1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. |
| Storage Buffer | 0.01M PBS, pH 7.4.Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
