Bioactivity
Detects IL2 in indirect ELISAs.FC
Flow-cytometry using anti-human IL2 antibody.THP-1 cells were stained with an irrelevant antibody or an anti-human IL2 antibody monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody and cells analysed on a NovoCyte Flow Cytometer.SDS-PAGE
SDS-PAGE for InVivoMAb Anti-Human IL2 .WB
Western blot analysis was performed using anti-human IL2 monoclonal antibody at 1ug/mL on various samples.Lane 1:recombinant human IL2 Lane 2:negative controlFC
Flow-cytometry using PE anti-human IL2 antibody. THP-1 cells were fixed and permeabilized, then stained with an irrelevant antibody or an PE anti-human IL2 monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.FC
Flow-cytometry using FITC anti-human IL2 antibody. THP-1 cells were fixed and permeabilized, then stained with an irrelevant antibody or an FITC anti-human IL2 monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.| Product Name | InVivoMAb Anti-Human IL-2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | V6N9 |
| Host Species | Mouse |
| Tested Applications | ELISAFCMNeutralization |
| ELISA:1:1000-1:10000 |
|
| Species Reactivity | Human |
| Predicted Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A/G |
| Target | IL2, IL-2, Aldesleukin, Interleukin-2, TCGF, T-cell growth factor |
|---|---|
| Function | InVivoMAb Anti-Human IL2 (Iv0020)[Iv0020] (HX011010) is amouse monoclonalantibody detectingIL2inELISA, FCM, Neutralization. Suitable forHuman.HighlightsMulti-Application— Validated across multiple applications.Flow Cytometry— Validated for flow cytometry applications. |
| SwissProt ID | P60568 |
|---|
Bioactivity
Detects IL2 in indirect ELISAs.
FC
Flow-cytometry using anti-human IL2 antibody.THP-1 cells were stained with an irrelevant antibody or an anti-human IL2 antibody monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody and cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE
SDS-PAGE for InVivoMAb Anti-Human IL2 .
WB
Western blot analysis was performed using anti-human IL2 monoclonal antibody at 1ug/mL on various samples.Lane 1:recombinant human IL2 Lane 2:negative control
FC
Flow-cytometry using PE anti-human IL2 antibody. THP-1 cells were fixed and permeabilized, then stained with an irrelevant antibody or an PE anti-human IL2 monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
FC
Flow-cytometry using FITC anti-human IL2 antibody. THP-1 cells were fixed and permeabilized, then stained with an irrelevant antibody or an FITC anti-human IL2 monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.| Application Notes | ELISA:1:1000-1:10000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. |
| Storage Buffer | 0.01M PBS, pH 7.4.Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
