Bioactivity
Detects CTSL in indirect ELISAs.FC
Flow-cytometry using anti-human CTSL antibody.CTSL Transfected CHO cells were stained with an irrelevant antibody or an anti-human CTSL antibody monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody and cells analysed on a NovoCyte Flow Cytometer.SDS-PAGE
SDS-PAGE for Anti-Human CTSL Antibody .WB
Western blot analysis was performed using anti-CTSL monoclonal antibody at 1ug/mL on various samples.Lane 1:recombinant human CTSL Lane 2:negative control| Product Name | Anti-Human CTSL Recombinant Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a,k |
| Clone Number | G4Z2 |
| Host Species | Mouse |
| Tested Applications | ELISAFCMIFIPWB |
| ELISA:1:2000-1:20000 WB:1:500-1:2000 IP:1:10-1:100 |
|
| Species Reactivity | Human |
| Predicted Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A/G |
| Target | CTSL1, MEP, Procathepsin L, Major excreted protein, Cathepsin L1, CTSL |
|---|---|
| Function | Anti-Human CTSL Antibody (3D8)[3D8] (HY099013) is amouse monoclonalantibody detectingCTSL1inELISA, FCM, IF, IP, WB. Suitable forHuman.HighlightsMulti-Application— Validated across multiple applications.Flow Cytometry— Validated for flow cytometry applications. |
| SwissProt ID | P07711 |
|---|
Bioactivity
Detects CTSL in indirect ELISAs.
FC
Flow-cytometry using anti-human CTSL antibody.CTSL Transfected CHO cells were stained with an irrelevant antibody or an anti-human CTSL antibody monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody and cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE
SDS-PAGE for Anti-Human CTSL Antibody .
WB
Western blot analysis was performed using anti-CTSL monoclonal antibody at 1ug/mL on various samples.Lane 1:recombinant human CTSL Lane 2:negative control| Application Notes | ELISA:1:2000-1:20000 WB:1:500-1:2000 IP:1:10-1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. |
| Storage Buffer | 0.01M PBS, pH 7.4.Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
